PMID: 11289269Apr 6, 2001Paper

Primary structure of kappa-casein isolated from mares' milk

The Journal of Dairy Research
B S IamettiF Bonomi

Abstract

In this work the purification and the complete primary structure of kappa-casein from equine milk are reported for the first time. Mares' milk casein was separated by RP-HPLC into four fractions. Complete primary sequence was obtained by sequence analysis of the protein in the fastest eluting peak isolated by chromatography. This sequence was 95% identical to that reported for the C-terminal portion of the zebras' kappa-casein and showed high similarity with kappa-caseins from sources other than Equidae, confirming that this protein was indeed kappa-casein in equine milk. The presence of post-translational modifications in equine kappa-casein was investigated by mass spectroscopy, after enzymic dephosphorylation. Two main components were found, the smaller component being more abundant. Equine kappa-casein was recognized by a lectin specific for one of the glucosidic bonds in the saccharide moiety of bovine kappa-casein. Sequence comparison with prevision studies showed that the distribution of charged and hydrophobic regions in equine kappa-casein was similar, but not identical, to that found in the bovine protein; these regions are associated with the role of kappa-casein in the formation and stabilization of the micellar str...Continue Reading

Citations

May 23, 2009·Rapid Communications in Mass Spectrometry : RCM·Vincenzo CunsoloSalvatore Foti
Mar 23, 2007·Rapid Communications in Mass Spectrometry : RCM·Vincenzo CunsoloSalvatore Foti
Jul 3, 2007·Journal of Mass Spectrometry : JMS·Vincenzo CunsoloSalvatore Foti
Sep 14, 2012·Journal of Mass Spectrometry : JMS·Rosaria SalettiSalvatore Foti
Oct 29, 2009·Journal of Mass Spectrometry : JMS·Vincenzo CunsoloSalvatore Foti
Apr 25, 2016·Journal of Dairy Science·J BrinkmannJ Tetens
Feb 19, 2013·Journal of Agricultural and Food Chemistry·Therese Uniacke-LoweDaniel M Mulvihill

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