PMID: 6986168Jan 22, 1980Paper

Primary structure of murine major histocompatibility complex alloantigens: amino acid sequence of the amino-terminal one hundred and seventy-three residues of the H-2Kb glycoprotein

Biochemistry
H UeharaT J Kindt

Abstract

The amino-terminal 173 residues of the murine histocompatibility antigen H-2Kb have been assigned by using radiochemical methodology. The complete sequence of an 86 residue glycopeptide (CN-Ib), which is one of the five major CNBr fragments of Kb, was determined by analysis of peptides obtained from digests using thrombin and V8 staphylococcal protease. Complete sequences were obtained for the three large thrombic peptides, and these were aligned by using peptides from the V8 protease digest. Alignment of the CNBr fragments was carried out by using [35S]Met-labeled peptides from a tryptic digest of the papain-cleaved H-2Kb molecule. Positive identification was possible for all the common amino acids except Asp (Asp) which was indirectly assigned and which is designated in italics. The sequence obtained in our studies was Gly-Pro-His-Ser-Leu-Arg-Tyr-Phe-Val-Thr-Ala-Val-Ser-Arg-Pro-Gly-Leu-Gly-Glu-Pro-Arg-Tyr-Met-Glu-Val-Gly-Tyr-Val-Asp-Asp-Thr-Glu-Phe-Val-Arg-Phe-Asp-Ser-Asp-Ala-Glu-Asn-Pro-Arg-Tyr-Glu-Pro-Arg-Ala-Arg-Trp-Met-Glu-Gln-Glu-Gly-Pro-Glu-Tyr-Trp-Glu-Arg-Glu-Thr-Gln-Lys-Ala-Lys-Gly-Asn-Glu-Gln-Ser-Phe-Arg-Val-Asp-Leu-Arg-Thr-Leu-Leu-Gly-Tyr-Tyr-(Asn)-Gln-Ser-Lys-Gly-Gly-Ser-His-Thr-Ile-Gln-Val-Ile-Ser-Gly-Cys-Glu-Val-...Continue Reading

References

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Citations

Jan 1, 1983·Immunogenetics·L R PeaseS G Nathenson
Jan 1, 1981·Immunogenetics·T NisizawaS G Nathenson
Jan 1, 1987·Immunologic Research·Y T ChenL J Old
Jan 1, 1981·Journal of Immunological Methods·J E Coligan, T J Kindt
Jul 1, 1991·Molecular Immunology·G M PfaffenbachD H Schulze
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J B RothbardB A Cunningham
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·F T GatesT J Kindt
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S KvistP Kourilsky
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·B Arden, J Klein
Aug 1, 1983·European Journal of Immunology·P Thammana, M D Scharff
Jan 1, 1980·Scandinavian Journal of Immunology·M S KrangelJ L Strominger
Apr 20, 2018·The FEBS Journal·Peter Parham
Jul 2, 1981·Nature·F BrégégèreB Dobberstein
May 7, 1981·Nature·J E ColiganS G Nathenson
Jan 1, 1980·Immunogenetics·B M EwensteinS G Nathenson

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