Primary structure of the hemoglobins from Sphenodon (Sphenodon punctatus, Tuatara, Rynchocephalia). Evidence for the expression of alpha D-gene

Biological Chemistry Hoppe-Seyler
A AbbasiG Braunitzer

Abstract

Sphenodon is the sole representative of the "beakhead" reptiles which were widely distributed during the Triassic period before the spectacular rise of dinosaurs. Sphenodon punctatus is the only survivor ("living fossil") of this period. The morphological features of Sphenodon are remarkably conservative and differ little from reptiles living 200 million years ago. In the present paper the determination of the primary structure of the tetrameric hemoglobins is described: three components are identified: hemoglobin A' (alpha A2 beta II2), hemoglobin A (alpha A2 beta I2) and hemoglobin D (alpha D2 beta II2). The components were characterized electrophoretically, the four different peptide chains were characterized by Triton electrophoresis as well as by high-performance liquid chromatography. The hemoglobins and--under dissociating conditions--also the chains, were isolated on columns of cellulose ion exchangers. Sequence determination was carried out after cleavage of the individual chains with trypsin and after a specific chemical cleavage of the Asp-Pro bond. For sequence determination the film technique and gas-phase method were employed. The data are compared with the sequence of the human hemoglobin, and interpretations of ...Continue Reading

Citations

Apr 1, 1991·Journal of Protein Chemistry·A Abbasi, Z H Zaidi
Oct 1, 1989·Journal of Protein Chemistry·A Abbasi, Z H Zaidi
Nov 1, 1994·Journal of Protein Chemistry·S NaqviZ H Zaidi
Jan 1, 1991·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·T Brittain
Mar 21, 2007·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Evaldo ReischlDanilo Wilhelm Filho
Sep 7, 2005·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Fumio ShishikuraTakatoshi Nagai
Sep 4, 1999·Clinical and Experimental Pharmacology & Physiology·R M Wells
Jan 9, 1999·The Journal of Biological Chemistry·C F BonafeM S Matsuura

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