PMID: 2120057Sep 24, 1990Paper

Primary structure of the oligo-1,6-glucosidase of Bacillus cereus ATCC7064 deduced from the nucleotide sequence of the cloned gene

European Journal of Biochemistry
K WatanabeY Suzuki

Abstract

The gene coding for Bacillus cereus ATCC7064 (mesophile) oligo-1,6-glucosidase was cloned within a 2.8-kb SalI-EcoRI fragment of DNA, using the plasmid pUC19 as a vector and Escherichia coli C600 as a host. E. coli C600 bearing the hybrid plasmid pBCE4 accumulated oligo-1,6-glucosidase in the cytoplasm. The cloned enzyme coincided absolutely with B. cereus oligo-1,6-glucosidase in its Mr (65,000), in its electrophoretic behavior on a polyacrylamide gel with or without sodium dodecyl sulfate, in its isoelectric point (4.5), in the temperature dependence of its stability and activity, and in its antigenic determinants. The nucleotide sequence of B. cereus oligo-1,6-glucosidase gene and its flanking regions was determined with both complementary strands of DNA (each 2838 nucleotides). The gene consisted of an open reading frame of 1674 bp commencing with a ATG start codon and followed by a TAA stop codon. The amino acid sequence deduced from the nucleotide sequence predicted a protein of 558 amino acid residues with a Mr of 66,010. The amino acid composition and Mr were comparable with those of B. cereus oligo-1,6-glucosidase. The predicted N-terminal sequence of 10 amino acid residues agreed completely with that of the cloned lig...Continue Reading

References

Jun 1, 1976·Applied and Environmental Microbiology·Y SuzukiS Abe
Jan 1, 1979·Annual Review of Genetics·M Rosenberg, D Court
Jan 1, 1978·Annual Review of Biochemistry·S Adhya, M Gottesman
Sep 15, 1977·Journal of Molecular Biology·P Y Chou, G D Fasman
May 1, 1987·Journal of Bacteriology·N KatsuragiY Murooka
Aug 1, 1988·Journal of Protein Chemistry·E A MacGregor
Jan 1, 1987·Methods in Enzymology·J Vieira, J Messing
Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·B W MatthewsW J Becktel
Jul 5, 1985·Journal of Molecular Biology·G von Heijne
Apr 20, 1973·Biochimica Et Biophysica Acta·P N LewisH A Scheraga
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J Shine, L Dalgarno
Aug 27, 1968·Journal of Chromatography·C W Wrigley
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D DiMaioT Maniatis
Jan 1, 1984·Applied and Environmental Microbiology·N F Paoni, R L Arroyo
Mar 1, 1984·Journal of Biochemistry·Y MatsuuraM Kakudo
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Dec 11, 1981·Science·F Sanger

❮ Previous
Next ❯

Citations

Jan 1, 1997·Progress in Biophysics and Molecular Biology·S Janecek
Dec 24, 1997·Comparative Biochemistry and Physiology. Part A, Physiology·D A Cowan
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·R G Wells, M A Hediger
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J BertranH Murer
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J BertranH Murer
Jan 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·X SunP Reichard
Oct 26, 2001·Bioscience, Biotechnology, and Biochemistry·K WatanabeY Suzuki
Jan 17, 1997·The Journal of Biological Chemistry·M Inohara-OchiaiY Shibano
Jan 1, 1994·FEMS Microbiology Letters·A P KellyD J McConnell

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.