Primary structure of Torpedo californica acetylcholinesterase deduced from its cDNA sequence

Nature
M SchumacherP Taylor

Abstract

Acetylcholinesterase, an essential enzyme of the nervous system, rapidly terminates the action of acetylcholine released into the synapse. Acetylcholinesterase is also found (in lower abundance) in extrajunctional areas of muscle and nerve and on erythrocyte membranes. Hydrodynamic analyses of the native enzyme and characterization of its dissociated subunits have revealed multiple enzyme forms which can be divided into two classes: dimensionally asymmetric forms which are usually found within the synapse and contain a collagen-like structural subunit disulphide-linked to the catalytic subunits; and globular forms which appear to be widely distributed on the outer surface of cell membranes. Both forms have been characterized in the ray Torpedo californica and, although their catalytic behaviours seem to be identical, they differ slightly in amino-acid composition, peptide maps and reactivity with certain monoclonal antibodies. Here, we report the complete amino-acid sequence of an acetylcholinesterase inferred from the sequence of a complementary DNA clone. The 575-residue protein shows significant homology with the C-terminal portion of thyroglobulin.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Feb 12, 1970·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·B S Hartley
Dec 1, 1969·European Journal of Biochemistry·J Massoulié, F Rieger
Jan 1, 1982·Annual Review of Neuroscience·J Massoulié, S Bon
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·B P DoctorP Taylor
Jan 1, 1983·Methods in Enzymology·J Messing
Sep 21, 1984·Science·J P ChangeuxP Chemouilli
Feb 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·T ClaudioS Heinemann
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle

❮ Previous
Next ❯

Citations

Apr 11, 2000·Microscopy Research and Technique·C Legay
Feb 15, 1997·The Journal of Experimental Zoology·D SutherlandL Pezzementi
Jan 1, 1996·Biopolymers·K H Mayo
Jun 15, 1994·Journal of Neuroscience Research·B CresnarJ Sketelj
Jan 1, 1996·Protein Science : a Publication of the Protein Society·I ShinL M Weiner
Jan 1, 1989·Archives of Toxicology·L FranciskovicE Reiner
Feb 1, 1991·Cellular and Molecular Neurobiology·P G Layer
Feb 1, 1991·Cellular and Molecular Neurobiology·L M Hall, C A Malcolm
Feb 1, 1990·Journal of Protein Chemistry·C WeiseF Hucho
Oct 1, 1990·The Journal of Membrane Biology·N C Inestrosa, A Perelman
Sep 1, 1996·Journal of Molecular Evolution·C RobinJ G Oakeshott
Jan 1, 1987·Molecular Neurobiology·H Soreq, A Gnatt
Jun 28, 2006·Cell and Tissue Research·Gabriel Zimmerman, Hermona Soreq
Nov 28, 1989·Biochimica Et Biophysica Acta·J A KisselD Y Hui
Feb 10, 1993·Biochimica Et Biophysica Acta·C S Wang, J A Hartsuck
Nov 1, 1990·Brain Research. Developmental Brain Research·C S Heck, P A McKinley
Nov 1, 1991·Trends in Pharmacological Sciences·F HuchoC Weise
Jul 1, 1992·Molecular and Biochemical Parasitology·C C Blackburn, M E Selkirk
Jun 15, 1992·Biochimica Et Biophysica Acta·S LonghiL Alberghina
Feb 20, 1993·Biochimica Et Biophysica Acta·N S ColwellB V Kumar
Aug 2, 1994·Biochimica Et Biophysica Acta·W R RandallN R Gough
Nov 9, 1989·Biochimica Et Biophysica Acta·C S PayneA D Wolfe
Mar 12, 1992·Biochimica Et Biophysica Acta·J K KruegerC E Schutt
Jan 1, 1990·Neurochemistry International·M D Cánovas-Muñoz, C J Vidal
Feb 1, 1989·Biochimie·Y MalthièryS Lissitzky
Nov 1, 1991·Biochimie·D AslanianJ Massoulié
Jul 1, 1993·Progress in Neurobiology·J MassouliéF M Vallette

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.