PMID: 6105959Jul 1, 1980Paper

Primary structure of triosephosphate isomerase from Bacillus stearothermophilus

European Journal of Biochemistry
S Artavanis-Tsakonas, J I Harris

Abstract

1. Triosephosphate isomerase from Bacillus stearothermophilus is a dimeric enzyme comprising two chemically identical polypeptide chains. 2. The nearly complete amino acid sequence of the subunit polypeptide chain has been established from sequences of tryptic, chymotryptic and lysine-blocked tyrptic fragments of S-[2-14C]carboxymethylated enzyme. Overlaps not established by experimental data have been provisionally established from considerations of sequence homology with previously established sequences for the rabbit, chicken and coelacanth enzymes. The nearly complete sequence of the 249 residues is as follows. (See Text). 3. Comparison of the thermophile and chicken muscle enzymes shows that 40% of the residues are in identical sequence. 4. Correlation of the sequence of the thermophile enzyme with the three-dimensional structure of the muscle enzyme shows that residues in the catalytic site and in the subunit interface are strongly conserved. Possible correlations between sequence changes and thermal stabilisation of the dimeric structure are also noted.

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Jan 1, 1984·Molecular & General Genetics : MGG·E PicherskyJ F Hess
Jan 1, 1984·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·J M NaiduH W Mohrenweiser
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Apr 1, 1997·Applied and Environmental Microbiology·R O TobiassenL Stepaniak

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