Primary support cultures of hippocampal and substantia nigra neurons.

Nature Protocols
Thomas FathLars M Ittner

Abstract

Primary cultures of rat and murine hippocampal neurons are widely used to reveal cellular mechanisms in neurobiology. Their use is limited, as culturing at low density is often not possible or is dependent on sophisticated methods. Here we present a novel method for culturing embryonic (E16.5) murine hippocampal neurons, using a spatially separated ring of cortical neurons for neurotrophic support. This method allows long-term cultures at a very low cell density, and therefore, the study of single embryo preparations and isolated neurons. This method has been adopted for neurons from the substantia nigra (E16.5), with support from a ring of striatal neurons.

References

May 13, 1977·Brain Research·G A Banker, W M Cowan
Jun 10, 1998·Neuron·M FischerA Matus
Mar 17, 2001·Nature Reviews. Molecular Cell Biology·J Massagué
Feb 14, 2002·Trends in Molecular Medicine·Pascal KurosinskiJürgen Götz
Jul 12, 2002·Journal of Neural Transmission·G GilleH Reichmann
Oct 14, 2003·Nature Neuroscience·Manuel Ackermann, Andrew Matus
Jun 23, 2004·Molecular and Cellular Neurosciences·Olivier RaineteauBeat H Gähwiler
Apr 20, 2005·The Journal of Cell Biology·Maurice KléberLukas Sommer
Oct 6, 2005·Neuron·Barbara Calabrese, Shelley Halpain
Jun 22, 2006·Journal of Neurochemistry·Juliane HamannGabriele Gille
Apr 5, 2007·Nature Protocols·Stefanie Kaech, Gary Banker
Jun 5, 2007·Nature Protocols·Lars M Ittner, Jürgen Götz
Oct 4, 2008·Proceedings of the National Academy of Sciences of the United States of America·Lars M IttnerJürgen Götz

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Citations

Jul 10, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Wenjun XieBianxiao Cui
Jul 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Janet van EerselLars M Ittner
Apr 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·Louise HedskogMaria Ankarcrona
Jun 6, 2009·The Journal of Biological Chemistry·Lars M IttnerJürgen Götz
Oct 16, 2012·Brain : a Journal of Neurology·Russell C DaleFabienne Brilot
Jun 17, 2010·PloS One·Nicole SchonrockJürgen Götz
Nov 30, 2012·Journal of Biomedicine & Biotechnology·Sui-Yi XuSu-Yue Pan
Oct 22, 2014·Proceedings of the National Academy of Sciences of the United States of America·Maria SepeAntonio Feliciello
Feb 18, 2016·Apoptosis : an International Journal on Programmed Cell Death·Guoqian HeChangqing Li
May 1, 2013·Stem Cells·Udo Schmidt-EdelkrautDietmar Spengler
Mar 10, 2016·The Journal of General Virology·Frédéric SorgeloosThomas Michiels
Sep 8, 2009·Biochimica Et Biophysica Acta·Birgitt SchüleJ William Langston
Sep 2, 2016·Environmental Science & Technology·Morgan M WillmingSandy Raimondo
Dec 24, 2014·Neurobiology of Aging·Pedro J ChacónAlfredo Rodríguez-Tébar
Nov 2, 2015·Ultramicroscopy·Takayuki UchihashiToshio Ando
Nov 5, 2017·The Journal of Biological Chemistry·Jennifer C PeelerThomas P Sakmar
Mar 5, 2015·Scientific Reports·Mikihiro ShibataRyohei Yasuda
Dec 18, 2013·Journal of Neural Transmission·Amadeus GladbachLars M Ittner

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