PMID: 8970955Dec 1, 1996Paper

Primary, syncytium-inducing human immunodeficiency virus type 1 isolates are dual-tropic and most can use either Lestr or CCR5 as coreceptors for virus entry

Journal of Virology
G SimmonsP R Clapham

Abstract

A panel of primary syncytium-inducing (SI) human immunodeficiency virus type 1 isolates that infected several CD4+ T-cell lines, including MT-2 and C8166, were tested for infection of blood-derived macrophages. Infectivity titers for C8166 cells and macrophages demonstrated that primary SI strains infected macrophages much more efficiently than T-cell line-adapted HIV-1 strains such as LAI and RF. These primary SI strains were therefore dual-tropic. Nine biological clones of two SI strains, prepared by limiting dilution, had macrophage/C8166 infectivity ratios similar to those of their parental viruses, indicating that the dual-tropic phenotype was not due to a mixture of non-SI/macrophage-tropic and SI/T-cell tropic viruses. We tested whether the primary SI strains used either Lestr (fusin) or CCR5 as coreceptors. Infection of cat CCC/CD4 cells transiently expressing Lestr supported infection by T-cell line-adapted strains including LAI, whereas CCC/CD4 cells expressing CCR5 were sensitive to primary non-SI strains as well as to the molecularly cloned strains SF-162 and JR-CSF. Several primary SI strains, as well as the molecularly cloned dual-tropic viruses 89.6 and GUN-1, infected both Lestr+ and CCR5+ CCC/CD4 cells. Thus, t...Continue Reading

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Citations

Mar 1, 1997·Nature Medicine·C PatienceR A Weiss
Mar 10, 1998·AIDS Research and Human Retroviruses·M R CapobianchiF Dianzani
Jan 7, 1998·Immunological Reviews·O J CohenA S Fauci
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·C OwmanR C Gallo
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Jun 8, 1999·Annual Review of Immunology·E A BergerJ M Farber
May 20, 1999·Molecular Membrane Biology·T L Hoffman, R W Doms
Aug 13, 1999·AIDS Research and Human Retroviruses·S HibbittsP R Clapham
Feb 26, 2000·Annual Review of Genetics·H TangF Wong-Staal
Mar 14, 2000·The Journal of Biological Chemistry·J ElsnerA E Proudfoot
Jul 20, 2001·AIDS Research and Human Retroviruses·F Salvatori, G Scarlatti
Nov 19, 2002·Clinical Chemistry and Laboratory Medicine : CCLM·Tonie Cilliers, Lynn Morris
Dec 19, 2002·AIDS Research and Human Retroviruses·Ping YeDenise E Kirschner
Sep 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·Janet M HarouseCecilia Cheng-Mayer
Jan 29, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Qi DaHong Tang
Mar 6, 2019·Nature·Ravindra K GuptaEduardo Olavarria
Sep 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·A KinterA S Fauci
Feb 3, 2000·Proceedings of the National Academy of Sciences of the United States of America·H BlaakH Schuitemaker

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