Primate immune responses to HIV-1 Env formulated in the saponin-based adjuvant AbISCO-100 in the presence or absence of TLR9 co-stimulation

Scientific Reports
Paola MartinezGunilla B Karlsson Hedestam

Abstract

Protein-based vaccines require adjuvants to achieve optimal responses. Toll-like receptor (TLR) 9 agonists were previously shown to improve responses to protein-based vaccines, such as the Hepatitis B virus vaccine formulated in alum. Here, we used CpG-C together with the clinically relevant saponin-based adjuvant AbISCO-100/Matrix-M (AbISCO), to assess if TLR9 co-stimulation would quantitatively or qualitatively modulate HIV-1 envelope glycoprotein (Env)-specific B and T cell responses in rhesus macaques. The macaques were inoculated with soluble Env trimers in AbISCO, with or without the addition of CpG-C, using an interval similar to the Hepatitis B virus vaccine. Following a comprehensive evaluation of antigen-specific responses in multiple immune compartments, we show that the Env-specific circulating IgG, memory B cells and plasma cells displayed similar kinetics and magnitude in the presence or absence of CpG-C and that there was no apparent difference between the two groups in the elicited HIV-1 neutralizing antibody titers or antigen-specific CD4+ T cell responses. Importantly, the control of SHIV viremia was significantly improved in animals from both Env-immunized groups relative to adjuvant alone controls, demonstra...Continue Reading

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Citations

Jul 23, 2016·Frontiers in Immunology·Paola Martinez-MurilloGunilla B Karlsson Hedestam
Jan 31, 2017·Immunological Reviews·Gunilla B Karlsson HedestamRichard T Wyatt
Jul 18, 2020·Journal of Leukocyte Biology·Li-Juan DengJia-Xu Chen
Mar 6, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ann J HessellNancy L Haigwood
Nov 11, 2019·The Journal of Experimental Medicine·Ganesh E PhadGunilla B Karlsson Hedestam
Sep 21, 2017·Frontiers in Immunology·Martina Soldemo, Gunilla B Karlsson Hedestam
Nov 28, 2018·Retrovirology·Jelle van Schooten, Marit J van Gils

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Methods Mentioned

BETA
lavages
flow cytometry
sedation
transfection
gel filtration
ELISA
FCS

Software Mentioned

Graph Pad Prism
JMP
AbISCO
FlowJo
Pestle

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