Prime-boost vaccination of mice and rhesus macaques with two novel adenovirus vectored COVID-19 vaccine candidates.

Emerging Microbes & Infections
Shengxue LuoChengyao Li


ABSTRACTCOVID-19 vaccines are being developed urgently worldwide. Here, we constructed two adenovirus vectored COVID-19 vaccine candidates of Sad23L-nCoV-S and Ad49L-nCoV-S carrying the full-length gene of SARS-CoV-2 spike protein. The immunogenicity of two vaccines was individually evaluated in mice. Specific immune responses were observed by priming in a dose-dependent manner, and stronger responses were obtained by boosting. Furthermore, five rhesus macaques were primed with 5 × 109 PFU Sad23L-nCoV-S, followed by boosting with 5 × 109 PFU Ad49L-nCoV-S at 4-week interval. Both mice and macaques well tolerated the vaccine inoculations without detectable clinical or pathologic changes. In macaques, prime-boost regimen induced high titers of 103.16 anti-S, 102.75 anti-RBD binding antibody and 102.38 pseudovirus neutralizing antibody (pNAb) at 2 months, while pNAb decreased gradually to 101.45 at 7 months post-priming. Robust T-cell response of IFN-γ (712.6 SFCs/106 cells), IL-2 (334 SFCs/106 cells) and intracellular IFN-γ in CD4+/CD8+ T cell (0.39%/0.55%) to S peptides were detected in vaccinated macaques. It was concluded that prime-boost immunization with Sad23L-nCoV-S and Ad49L-nCoV-S can safely elicit strong immunity in anim...Continue Reading


Jan 20, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Scott E HensleyHildegund C J Ertl
Jan 6, 2012·Science Translational Medicine·Eleanor BarnesPaul Klenerman
Nov 21, 2014·Journal of Virology·Peter AbbinkDan H Barouch
Dec 23, 2015·Vaccine·Kimia KardaniSepideh Shahbazi
Feb 3, 2016·Annals of Internal Medicine·Lindsey R BadenUNKNOWN B003-IPCAVD004-HVTN091 Study Group
Jan 28, 2020·Lancet·Chen WangGeorge F Gao
Feb 29, 2020·Scientific Reports·Brianna L BullardEric A Weaver
Mar 11, 2020·Cell·Alexandra C WallsDavid Veesler
Apr 8, 2020·Immunity·Fatima Amanat, Florian Krammer
May 22, 2020·Science·Jingyou YuDan H Barouch
Jul 15, 2020·The New England Journal of Medicine·Lisa A JacksonUNKNOWN mRNA-1273 Study Group
Jul 30, 2020·The New England Journal of Medicine·Kizzmekia S CorbettBarney S Graham
Jul 31, 2020·Nature·Noe B MercadoDan H Barouch
Jul 31, 2020·Nature·Neeltje van DoremalenVincent J Munster
Aug 14, 2020·Nature·Mark J MulliganKathrin U Jansen
Aug 18, 2020·Virus Research·Simran Preet Kaur, Vandana Gupta
Sep 5, 2020·Nature Medicine·Lisa H TostanoskiDan H Barouch
Jan 14, 2021·The New England Journal of Medicine·Jerald SadoffHanneke Schuitemaker

❮ Previous
Next ❯


Sep 23, 2021·Comparative Medicine·Anita M Trichel

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.