PMID: 11607444Dec 15, 1993Paper

Primer-mediated in situ detection of the B-hordein gene cluster on barley chromosome 1H

Proceedings of the National Academy of Sciences of the United States of America
S AbboI P King

Abstract

In situ hybridization methods allow the detection of specific DNA sequences on whole chromosomes. The technique has been widely used as a diagnostic and research tool by animal cytogeneticists, for whom detection of unique sequences on mammalian chromosomes is routinely achieved. However, detection of unique sequences on plant chromosomes is less reliable. The recently developed primer-induced in situ hybridization (PRINS) technique allows rapid and reliable in situ detection by the hybridization of primers to denatured target DNA, followed by extension with DNA polymerase in the presence of a labeled nucleotide. The use of short oligonucleotide primers could allow improved penetration of debris and highly condensed chromatin common in preparations of plant chromosomes, thus increasing the sensitivity of in situ detection. The feasibility of this approach is demonstrated by the oligonucleotide primer-mediated detection of the B-hordein gene cluster on a barley chromosome. Applications of the PRINS technique for plant cytogeneticists are discussed.

References

Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J P Gustafson, J E Dillé
Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J E WernerB S Gill
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J P GustafsonC L McIntyre
Jan 1, 1985·Journal of Cell Science. Supplement·K K Mills, W D Bauer

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Citations

Jan 1, 1995·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·S AbboG Moore
Apr 1, 1996·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·H M ThomasJ A Harper
Sep 7, 2006·Cytogenetic and Genome Research·F PellestorS Hamamah
Jan 25, 2002·American Journal of Medical Genetics·Franck PellestorBrigitte Andréo
May 7, 2009·FEMS Yeast Research·Maciej WnukEwa Slota

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