Primer sets developed to amplify conserved genes from filamentous ascomycetes are useful in differentiating fusarium species associated with conifers.

Applied and Environmental Microbiology
G C DonaldsonN L Glass

Abstract

We examined the usefulness of primer sets designed to amplify introns within conserved genes in filamentous ascomycetes to differentiate 35 isolates representing six different species of Fusarium commonly found in association with conifer seedlings. We analyzed restriction fragment length polymorphisms (RFLP) in five amplified PCR products from each Fusarium isolate. The primers used in this study were constructed on the basis of sequence information from the H3, H4, and (beta)-tubulin genes in Neurospora crassa. Primers previously developed for the intergenic transcribed spacer region of the ribosomal DNA were also used. The degree of interspecific polymorphism observed in the PCR products from the six Fusarium species allowed differentiation by a limited number of amplifications and restriction endonuclease digestions. The level of intraspecific RFLP variation in the five PCR products was low in both Fusarium proliferatum and F. avenaceum but was high in a population sample of F. oxysporum isolates. Clustering of the 35 isolates by statistical analyses gave similar dendrograms for H3, H4, and (beta)-tubulin RFLP analysis, but a dendrogram produced by intergenic transcribed spacer analysis varied in the placement of some F. ox...Continue Reading

Citations

Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Keith A SeifertPaul D N Hebert
Feb 15, 2003·FEMS Microbiology Letters·Prashant K MishraAlastair Culham
Mar 29, 2002·Molecular Biology and Evolution·Marie-Josée DaboussiThierry Langin
Nov 1, 2003·Mycologia·Meredith BlackwellKevin G Jones
Jul 31, 1999·Applied and Environmental Microbiology·E T SteenkampW F Marasas
Apr 1, 1995·Applied and Environmental Microbiology·N L Glass, G C Donaldson
Apr 5, 2003·Journal of Invertebrate Pathology·Midori SugimotoHideyuki Nagao
Nov 2, 2006·Mycological Research·Soon Gyu HongAlessandra Belisario

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