PMID: 11907161Mar 22, 2002Paper

Priming of alveolar macrophage respiratory burst by H(2)O(2) is prevented by phosphatidylcholine-specific phospholipase C inhibitor Tricyclodecan-9-yl-xanthate (D609)

The Journal of Pharmacology and Experimental Therapeutics
Julio Girón-CalleH J Forman

Abstract

The respiratory burst in alveolar macrophages is enhanced in vitro by pre-exposure to nontoxic concentrations of hydroperoxides before stimulation by an agonist, which may represent a feed-forward regulatory mechanism. Tricyclodecan-9-yl-xanthate (D609), an inhibitor of phosphatidylcholine-specific phospholipase C (PC-PLC), suppresses this priming of the respiratory burst by pre-exposure to H(2)O(2) in NR8383 alveolar macrophages (up to 100 microM D609, 400 nmol of H(2)O(2) added to 5 x 10(6) cells 15 min before stimulation with ADP). D609 has potential as an antioxidant due to its dithiocarbonate functional group that allows it to slowly react with H(2)O(2) and rapidly reduce cytochrome c, which interferes with a common assay for the respiratory burst. Nonetheless, the antioxidant properties of D609 do not account for its inhibition of priming of the respiratory burst by H(2)O(2). Reduction of nitro blue tetrazolium is the basis for an assay for superoxide production with which D609 does not interfere. With this assay, it was found that D609 does not inhibit the respiratory burst per se, but prevents its enhancement by pre-exposure to H(2)O(2). Consistent with a role of diacylglycerol generation by phospholipase C, this enhanc...Continue Reading

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Citations

Nov 22, 2011·Neurochemical Research·Rao Muralikrishna AdibhatlaA Gusain
Dec 13, 2006·American Journal of Respiratory Cell and Molecular Biology·Honglei LiuHenry Jay Forman
Jul 12, 2011·Free Radical Biology & Medicine·Gayatri PremasekharanHenry Jay Forman
Jan 28, 2014·PloS One·Anna A PimenovaIna Tesseur
May 14, 2005·Brain Research. Brain Research Reviews·Chava B PocernichD Allan Butterfield
Sep 18, 2003·Journal of Lipid Research·Ryan J Perry, Neale D Ridgway
Jan 1, 2013·Molecular Neurobiology·Haviryaji S G KalluriRobert J Dempsey
Nov 5, 2019·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Kamila PrymasKatarzyna Kwiatkowska
Apr 4, 2021·Journal of Clinical Medicine·María MagallónFrancisco Dasí

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