PMID: 6160875Dec 2, 1980Paper

Priming of the fluid pump by osmotic gradients across rabbit corneal endothelium

Biochimica Et Biophysica Acta
J FischbargR Koatz

Abstract

The present study shows that the inclusion of 5% Dextran (average mol. wt. 40 000) in solutions to preserve in vitro rabbit corneal endothelium induces a sizable osmotic flow across the preparation which is superimposed on the existing fluid transport. Furthermore, even after fluid transport ceases due to in vitro deterioration, the Dextran-induced flow remains for some addition time. The osmotic permeability was 162 +/- 17 micrometer/s in the presence of glucose and 451 +/- 84 micrometer/s in its absence. The latter, comparatively high value suggests that such osmotic flow traverses the intracellular junctions. In addition, temporary (10--15 min) imposition of an osmotic gradient has a separate stimulatory 'priming' effect on the rate of fluid transport. Thus, the rate of fluid pumping increased by about 40% after challenge with Dextran. It was further noted that, after addition of Dextran, preparations in the absence of glucose escape gross deterioration for a time longer than those in the presence of glucose. On the other hand, mere addition of Dextran to a glucose-containing solution does not appear to prolong the estimated 'survival time' of the pumping mechanism. The sizable osmotic flows and the priming effect described ...Continue Reading

References

Feb 23, 1978·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·A E Hill, B S Hill
Jun 30, 1977·The Journal of Membrane Biology·J FischbargJ Bourguet
Dec 1, 1975·The Journal of General Physiology·H Sackin, E L Boulpaep
May 10, 1973·Experimental Eye Research·G I KayeF B Hoefle
May 10, 1973·Experimental Eye Research·J Fischbarg
Jan 1, 1967·Experimental Eye Research·S Mishima, B O Hedbys

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Citations

Feb 8, 2003·Experimental Eye Research·Jeffrey W Ruberti, Stephen D Klyce
Jan 1, 1981·Journal - American Intra-Ocular Implant Society·C F Bahn, A Sugar
Apr 1, 1985·Current Eye Research·D M Maurice

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