Priming with inflammatory cytokines is not a prerequisite to increase immune-suppressive effects and responsiveness of equine amniotic mesenchymal stromal cells.

Stem Cell Research & Therapy
Anna Lange-ConsiglioOrnella Parolini

Abstract

Equine amniotic mesenchymal stromal cells (AMSCs) and their conditioned medium (CM) were evaluated for their ability to inhibit in vitro proliferation of peripheral blood mononuclear cells (PBMCs) with and without priming. Additionally, AMSC immunogenicity was assessed by expression of MHCI and MHCII and their ability to counteract the in vitro inflammatory process. Horse PBMC proliferation was induced with phytohemagglutinin. AMSC priming was performed with 10 ng/ml of TNF-α, 100 ng/ml of IFN-γ, and a combination of 5 ng/ml of TNF-α and 50 ng/ml of IFN-γ. The CM generated from naïve unprimed and primed AMSCs was also tested to evaluate its effects on equine endometrial cells in an in vitro inflammatory model induced by LPS. Immunogenicity marker expression (MHCI and II) was evaluated by qRT-PCR and by flow cytometry. Priming does not increase MHCI and II expression. Furthermore, the inhibition of PBMC proliferation was comparable between naïve and conditioned cells, with the exception of AMSCs primed with both TNF-α and IFN-γ that had a reduced capacity to inhibit T cell proliferation. However, AMSC viability was lower after priming than under other experimental conditions. CM from naïve and primed AMSCs strongly inhibited PBM...Continue Reading

References

Dec 1, 1996·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M PootR P Haugland
Oct 19, 2001·American Journal of Perinatology·G Gaunt, K Ramin
Feb 2, 2002·Experimental Hematology·Amelia BartholomewRonald Hoffman
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
May 12, 2005·BMC Biotechnology·Deborah RibbleYiqun G Shellman
Jul 18, 2006·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Victor Y L LeungKenneth M C Cheung
Aug 22, 2008·Experimental Hematology·Wing Keung ChanGodfrey Chi-Fung Chan
Dec 18, 2008·Reproductive Biology and Endocrinology : RB&E·Gaetano DonofrioIain Martin Sheldon
Mar 20, 2010·Birth Defects Research. Part C, Embryo Today : Reviews·Caren E Petrie Aronin, Rocky S Tuan
Feb 22, 2011·Analytical and Bioanalytical Chemistry·Andrey V KuznetsovJakob Troppmair
Jul 19, 2011·Journal of Biomedical Science·Pei-Min ChenB-Linju Yen
Sep 29, 2011·Journal of Tissue Engineering and Regenerative Medicine·A Lange-ConsiglioF Cremonesi
Jan 10, 2012·Trends in Immunology·Yufang ShiGuangwen Ren
Oct 24, 2012·Immunology and Cell Biology·Karen English
Apr 19, 2015·Reproductive Biology and Endocrinology : RB&E·Bence SomoskoiSandor Cseh
Jun 27, 2017·American Journal of Veterinary Research·Jacqueline A HillLisa A Fortier
Aug 26, 2017·International Journal of Molecular Sciences·Francisco J VizosoRoman Perez-Fernandez
Nov 29, 2017·Stem Cells International·Cosette M Rivera-CruzMarxa L Figueiredo
Dec 19, 2017·Veterinary Immunology and Immunopathology·Jennifer M CassanoLisa A Fortier
May 18, 2018·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Catriona J CunninghamStuart M Allan
May 3, 2019·Stem Cell Research & Therapy·Nádia de Cássia NoronhaKelen C R Malmegrim
May 11, 2019·Journal of Clinical Medicine·Mehdi NajarLaurence Lagneaux

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Citations

Feb 2, 2021·Frontiers in Bioengineering and Biotechnology·Andrea PapaitOrnella Parolini

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Methods Mentioned

BETA
reverse transcription-PCR
PCR
flow cytometry
density gradient centrifugation
blood collection

Software Mentioned

GraphPad Prism
PerlPrimer
ImageJ
Weasel
GraphPad

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