Priming with Toll-like receptor 3 agonist or interferon-gamma enhances the therapeutic effects of human mesenchymal stem cells in a murine model of atopic dermatitis

Stem Cell Research & Therapy
Arum ParkJinho Yu

Abstract

Atopic dermatitis (AD) is a chronic and relapsing inflammatory skin disease. Great efforts have been recently made to treat AD using mesenchymal stem cells (MSCs), which have immunomodulatory functions. However, the immunomodulatory effects of MSCs need to be enhanced for clinical application in the treatment of AD. To evaluate and characterise the therapeutic effects of human Wharton's jelly-derived MSCs (WJ-MSCs) primed with the Toll-like receptor 3 agonist poly I:C or interferon-γ (IFN-γ) in a murine model of AD. Mice were treated with Aspergillus fumigatus extract to induce AD and then subcutaneously injected with non-primed, poly I:C-primed or IFN-γ-primed WJ-MSCs. Clinical symptom scores, transepidermal water loss (TEWL), histological characteristics and cytokine levels were determined. Transcriptome profiling and pathway analyses of primed WJ-MSCs were conducted. The clinical symptom score and TEWL in skin lesions were reduced in mice administered non-primed and primed WJ-MSCs. Epidermal thickness and inflammatory cell infiltration in skin lesions were reduced more in mice administered primed WJ-MSCs than in mice administered non-primed WJ-MSCs. Secretion of interleukin-17 was significantly reduced in skin draining lymph...Continue Reading

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Citations

Jun 20, 2020·Journal of Cellular Physiology·Hossein AbbaszadehKarim Shamsasenjan
Jun 2, 2020·Frontiers in Cell and Developmental Biology·Sérgio Ricardo Teixeira DaltroMilena Botelho Pereira Soares
Mar 27, 2021·Frontiers in Cell and Developmental Biology·Álvaro Sierra-SánchezSalvador Arias-Santiago

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
chips
ELISAs
enzyme-linked immunosorbent assays

Software Mentioned

Optix MX3
SAS
Ingenuity Pathway Analysis ( IPA )
R
Linear Models for Microarray
IMT i - Solution
IPA

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Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

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Aspergillosis (ASM)

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