Primordial germ cell-derived embryonic germ cells of the mouse-in vitro model for cytotoxicity studies with chemical mutagens

Toxicology in Vitro : an International Journal Published in Association with BIBRA
U SehlmeyerA M Wobus

Abstract

Different screening methods to detect the toxic effects of xenobiotics using cells from vertebrates and invertebrates in cytotoxicity and viability assays have been developed, but up to now appropriate in vitro methods with mammalian germ cells have not been available. In the present study the primordial germ (PG) cell-derived permanent embryonic germ (EG) cell line EG-1 was used as in vitro model in toxicity studies with chemical mutagens. EG-1 cells and embryonic stem cells of line D3 were comparatively investigated for their cell survival in response to N-ethyl-N-nitrosourea (ENU), N-methyl-N-nitro-N-nitrosoguanidine (MNNG) and mitomycin C (MMC) and the results compared with those obtained for undifferentiated embryonic carcinoma cells of line P19 and differentiated epithelioid EPI-7 cells. As a prerequisite for in vitro toxicity and viability studies the cultivation conditions for EG-1 and D3 cells in the absence of a feeder layer were improved by a conditioned medium, increasing the plating efficiency from 0.08% to 17.5% and from 21.1% to 25.1% for EG-1 and D3 cells, respectively. The resulting mean generation time (MGT) of 16.9 hr for EG-1 cells was identical to the generation time of PG cells in vivo, and was not signifi...Continue Reading

References

Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·D Solter, B B Knowles
Apr 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·G R Martin, M J Evans
Mar 1, 1989·Mutation Research·R Ghosh, S B Bhattacharjee
Apr 1, 1987·Mutation Research·R Goth-Goldstein, M Hughes
Apr 1, 1987·The Journal of Experimental Zoology·J R McCarreyJ L Bolen
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·E G BerstineB Ephrussi
Jan 1, 1982·Teratogenesis, Carcinogenesis, and Mutagenesis·G L KimmelR M Pratt
Aug 15, 1980·Science·G R Martin
Dec 1, 1982·Experimental Cell Research·M De Felici, A McLaren
May 1, 1984·Experimental Cell Research·A M WobusJ Schöneich
Apr 1, 1983·Experimental Cell Research·M De Felici, A McLaren
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·G R Martin
Feb 1, 1982·Developmental Biology·M W McBurney, B J Rogers
Jan 1, 1993·Methods in Enzymology·M V Wiles
Jan 1, 1993·Methods in Enzymology·J E CookeC C Wylie
Feb 1, 1994·Developmental Biology·C L StewartH Bhatt
Jan 1, 1993·Human Reproduction·M L Meistrich
Jan 1, 1988·Toxicology in Vitro : an International Journal Published in Association with BIBRA·E BorenfreundN Martin-Alguacil

❮ Previous
Next ❯

Citations

Jan 13, 2011·Archives of Toxicology·Anna M Wobus, Peter Löser
Nov 8, 2001·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J RohwedelA M Wobus

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.