Primordial oscillations in life: Direct observation of glycolytic oscillations in individual HeLa cervical cancer cells

Chaos
Takashi AmemiyaTomohiko Yamaguchi

Abstract

We report the first direct observation of glycolytic oscillations in HeLa cervical cancer cells, which we regard as primordial oscillations preserved in living cells. HeLa cells starved of glucose or both glucose and serum exhibited glycolytic oscillations in nicotinamide adenine dinucleotide (NADH), exhibiting asynchronous intercellular behaviors. Also found were spatially homogeneous and inhomogeneous intracellular NADH oscillations in the individual cells. Our results demonstrate that starved HeLa cells may be induced to exhibit glycolytic oscillations by either high-uptake of glucose or the enhancement of a glycolytic pathway (Crabtree effect or the Warburg effect), or both. Their asynchronous collective behaviors in the oscillations were probably due to a weak intercellular coupling. Elucidation of the relationship between the mechanism of glycolytic dynamics in cancer cells and their pathophysiological characteristics remains a challenge in future.

References

Jul 2, 1973·Biochemical and Biophysical Research Communications·S N Shaw, H Amos
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·Y Termonia, J Ross
Dec 2, 1999·Nature·S DanøF Hynne
May 4, 2000·Molecular Biology of the Cell·K PaemeleireM J Sanderson
Mar 30, 2002·Science·George M Whitesides, Bartosz Grzybowski
Feb 24, 1956·Science·O WARBURG
Feb 6, 2004·Journal of Cell Science·George C LinThomas H Steinberg
Apr 2, 2005·Nature Reviews. Neuroscience·Alfons Schnitzler, Joachim Gross
Jan 1, 1929·The Biochemical Journal·H G Crabtree
Nov 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Silvia De MontePreben Graae Sørensen
Oct 25, 2008·The Journal of Biological Chemistry·Jun-Hai YangJames N Weiss
May 23, 2009·Science·Matthew G Vander HeidenCraig B Thompson
Oct 20, 2009·Bio Systems·Jana Schütze, Jana Wolf
Apr 24, 2010·Science·Thomas GregorSatoshi Sawai
Jul 9, 2011·Science·Fiona A ChandraJohn C Doyle
Feb 15, 2012·The Journal of Experimental Medicine·Robert B Hamanaka, Navdeep S Chandel
May 23, 2012·The FEBS Journal·Anna-Karin GustavssonJacky L Snoep
Sep 18, 2012·PloS One·André WeberMarcus J B Hauser
Apr 1, 2014·Trends in Endocrinology and Metabolism : TEM·Richard K P Benninger, David W Piston
Nov 19, 2015·Physics in Medicine and Biology·Leonard Che FruMichael W Kissick

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Citations

May 22, 2018·The FEBS Journal·Kenichi ShibataTomohiko Yamaguchi
Nov 5, 2019·Frontiers in Neuroscience·Jose L Perez VelazquezRamon Guevara Erra
Mar 5, 2020·Journal of Biological Physics·Jose Luis Perez Velazquez
Feb 16, 2021·Frontiers in Physiology·Joe Rowland Adams, Aneta Stefanovska
Oct 12, 2021·Current Genetics·Marcus J B Hauser

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