Principal frequency component analysis based on modulate chopper technique used in diffuse reflectance spectroscopy measurement

Applied Optics
Shaoxiu SongLing Lin

Abstract

Diffuse reflectance spectroscopy (DRS) is significantly affected from the interference of the ambient light and dark current of the instrument. Optical choppers, together with lock-in/synchronous amplification, can overcome these interferences. However, in spectral measurement, the sampling rate of the spectrometer is different from the Δ-pulse sampling, which is not high enough because of the integration time. In addition, the energy distribution is not perfectly concentrated as expected in modulate chopper technology. Therefore, in this study, based on the modulate chopper technique, we proposed a principal frequency component analysis (PFCA) method for DRS. This technique not only effectively eliminated the interference and dark current of the instrument but also improved the measurement precision using the energy of different frequencies. First, experiments were designed to successfully verify the function of optical choppers, eliminating the interference of the ambient light. Second, a set of 64 mixture solutions was designed and measured by DRS using the PFCA method to prove the feasibility of the proposed method. The solution was mixed with intralipid-20% suspension, India ink, and rhodamine B. These samples were analyze...Continue Reading

References

Feb 15, 1986·Applied Optics·A KrabbeK Proetel
Oct 16, 2008·Analytical and Bioanalytical Chemistry·M BlancoM Bautista
May 15, 2007·Talanta·J LuypaertY Vander Heyden
Aug 1, 1981·Applied Optics·J Priu, W Budde
Mar 8, 2013·The Review of Scientific Instruments·Gang LiLing Lin

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