Principles and applications of PRINS in cytogenetics

Current Protocols in Human Genetics
J Koch

Abstract

A flexible, low-cost alternative to FISH, primed in situ labeling (PRINS) has traditionally been used to detect tandemly repeated target sequences in chromosomes and nuclei. The technique is capable of discriminating among closely related DNA sequences in situ and has the advantage of using very small probes which easily penetrate to almost any target. This unit describes basic PRINS and the alternative version, dideoxy-PRINS, which can increase the sensitivity of the reaction by an order of magnitude. New material on multicolor PRINS and quantitative PRINS has been added. Protocols for detection of single-copy sequences and for application to the study of in-vivo activity of DNA-modifying enzymes are planned.

References

Jan 1, 1995·Cytogenetics and Cell Genetics·A J TherkelsenS Kølvraa
May 8, 1995·American Journal of Medical Genetics·F PellestorJ P Charlieu
Jan 1, 1995·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·J HindkjaerL Bolund
Mar 3, 1999·Nature Biotechnology·N Serakinci, J Koch
Nov 27, 1999·Cytogenetics and Cell Genetics·N SerakinciJ Koch
Nov 14, 2000·American Journal of Medical Genetics·J S KadandaleA T Tharapel
Nov 22, 2000·Microbial & Comparative Genomics·J S KadandaleA T Tharapel
Aug 30, 2002·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Claus L AndersenJørn Koch
Nov 7, 2002·Cancer Genetics and Cytogenetics·Nedime SerakinciJørn Koch

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Citations

Aug 2, 2011·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Joseph J VolpePaul A Rosenberg
Mar 9, 2011·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Joseph J VolpePaul A Rosenberg

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