Principles and approaches for reproducible scoring of tissue stains in research

Laboratory Investigation; a Journal of Technical Methods and Pathology
David K Meyerholz, Amanda P Beck

Abstract

Evaluation of tissues is a common and important aspect of translational research studies. Labeling techniques such as immunohistochemistry can stain cells/tissues to enhance identification of specific cell types, cellular activation states, and protein expression. While qualitative evaluation of labeled tissues has merit, use of semiquantitative and quantitative scoring approaches can greatly enhance the rigor of the tissue data. Adhering to key principles for reproducible scoring can enhance the quality and reproducibility of the tissue data so as to maximize its biological relevance and scientific impact.

References

Sep 1, 1988·American Journal of Clinical Pathology·L D True
May 1, 1996·American Journal of Respiratory Cell and Molecular Biology·D I BlythD Fattah
Oct 8, 1998·Histopathology·S S Cross
Mar 14, 2002·Toxicologic Pathology·Cynthia ShackelfordRonald Herbert
Oct 10, 2002·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·K KumagaiK Shirato
Oct 23, 2002·ILAR Journal·Michael F W Festing
Jan 10, 2004·Toxicologic Pathology·James W CrissmanDaryl C Thake
Jan 28, 2005·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Andre BleichJohn P Sundberg
Aug 11, 2006·Nature Biotechnology·David L Rimm
Jun 15, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·David L RimmBarbara Burtness
Feb 22, 2008·Journal of Veterinary Medical Education·Fred R Dee, David K Meyerholz
Jun 25, 2008·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Simone P ZehntnerBarry J Bedell
Jul 22, 2009·Infection and Immunity·Philana Ling LinJoAnne L Flynn
Aug 19, 2009·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Kingshuk Roy ChoudhuryJoseph G Rajendran
Jan 1, 2009·Biomedical Informatics Insights·David E AxelrodJudith-Anne W Chapman
Apr 3, 2010·BioTechniques·Jennifer BordeauxDavid Rimm
Jul 14, 2010·American Journal of Respiratory and Critical Care Medicine·David K MeyerholzMichael J Welsh
Mar 18, 2011·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Ran XieStephen M Hewitt
Aug 10, 2011·PloS One·Lee A BorthwickAndrew J Fisher
Sep 21, 2011·Veterinary Pathology·C J ZeissH G Allore
Feb 4, 2012·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·James A KrausRohit Bhargava
Mar 24, 2012·The American Journal of Dermatopathology·Sara C ShalinAlexander J Lazar
Mar 27, 2012·Journal of Clinical Pathology·Christopher FioreRichard Flavin
Apr 19, 2012·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Chibeza C AgleyStephen D R Harridge
Aug 21, 2012·Journal of Histotechnology·Alicia K OlivierDavid K Meyerholz
Nov 20, 2012·American Journal of Clinical Pathology·David A CohenRohit Bhargava
Feb 6, 2013·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Ellen C Jensen
Feb 13, 2013·Laboratory Investigation; a Journal of Technical Methods and Pathology·Robert D CardiffAlexander D Borowsky
Apr 6, 2013·Veterinary Pathology·K N Gibson-CorleyD K Meyerholz
Aug 24, 2013·Pathobiology of Aging & Age Related Diseases·Warren LadigesPiper M Treuting
Oct 31, 2013·American Journal of Respiratory and Critical Care Medicine·Ryan J AdamDavid A Stoltz

❮ Previous
Next ❯

Citations

Aug 23, 2018·Veterinary Pathology·David K MeyerholzAmanda P Beck
Oct 4, 2018·Veterinary Pathology·Laura J JankePeter Vogel
Oct 30, 2018·Veterinary Pathology·Krista M D La Perle
Jan 30, 2019·Journal of Leukocyte Biology·Ramasatyaveni GeesalaThorsten Maretzky
Feb 5, 2019·ILAR Journal·David K Meyerholz, Amanda P Beck
Sep 19, 2019·Cells·Gerd JomrichSebastian F Schoppmann
Oct 26, 2018·BMC Research Notes·David K MeyerholzHeather A Flaherty
Mar 11, 2020·EMBO Molecular Medicine·Zdeněk DvořákSridhar Mani
Mar 7, 2019·The Journal of Experimental Medicine·Shaojun XingHai-Hui Xue
Sep 20, 2018·Veterinary Pathology·Jeff L CaswellJyoji Yamate
Jul 25, 2019·Cell Death & Disease·Caterina IeranòStefania Scala
Mar 5, 2020·Cell and Tissue Research·Amanda P Beck, David K Meyerholz
Aug 5, 2020·Science Translational Medicine·Athri D RathnayakeKyeong-Ok Chang
Jul 30, 2019·The Journal of Clinical Investigation·Rudragouda ChannappanavarStanley Perlman
Aug 30, 2019·Journal of Applied Physiology·Scott M EbertChristopher M Adams
Nov 23, 2019·Respiratory Research·Kalina R Atanasova, Leah R Reznikov
Feb 27, 2020·Hepatology International·H TaghizadehG W Prager
Mar 21, 2020·Scientific Reports·Johanna UthoffJessica C Sieren
May 15, 2019·Applied Immunohistochemistry & Molecular Morphology : AIMM·Elizabeth A ChlipalaBrad Bolon
Oct 8, 2020·ELife·Lynda S OstedgaardMichael J Welsh
Oct 21, 2020·Journal of Gynecologic Oncology·Jee Suk ChangYong Bae Kim
Nov 12, 2020·Revista Do Instituto De Medicina Tropical De São Paulo·Silvia D'Andretta IgleziasThales De Brito
Nov 20, 2020·Veterinary Pathology·Nina OstfeldNicole Borel
Dec 18, 2020·Veterinary Pathology·Mark James HoenerhoffAmanda P Beck
Feb 23, 2020·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jordan L KohlmeyerDawn E Quelle
Jul 2, 2020·The Korean Journal of Internal Medicine·Guk Jin LeeIn Sook Woo
Mar 5, 2021·Journal of Cutaneous Pathology·Ahmed K AlomariRachel P Kowal
Apr 29, 2021·Veterinary Pathology·David K MeyerholzAlessandra Piersigilli
Jun 5, 2021·Scientific Reports·Buyanbileg Sodnom-IshSoung Min Kim
Jun 9, 2021·Proceedings of the National Academy of Sciences of the United States of America·Lok-Yin Roy WongStanley Perlman
Jun 3, 2021·Cancers·Justyna M PrzystalGhazaleh Tabatabai
Jul 6, 2021·Frontiers in Immunology·Adrianos NezosClio P Mavragani
Jul 3, 2021·Biomedicines·Chandra K MaharjanDawn E Quelle
Jul 22, 2021·Physiological Reports·Keyan ZareiDavid A Stoltz
Aug 21, 2021·Journal of Gastroenterology·Ingunn BakkeAnn Elisabet Østvik

❮ Previous
Next ❯

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
biopsy

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Brazilian Oral Research
Carlos Estrela, Altair Antoninha Del Bel Cury
The Journal of the Association of Nurses in AIDS Care : JANAC
Nancy C Sharts-Hopko
Journal for Specialists in Pediatric Nursing : JSPN
Eileen Thomas, Joan K Magilvy
© 2022 Meta ULC. All rights reserved