Principles of carbon catabolite repression in the rice blast fungus: Tps1, Nmr1-3, and a MATE-family pump regulate glucose metabolism during infection.

PLoS Genetics
J FernandezR A Wilson

Abstract

Understanding the genetic pathways that regulate how pathogenic fungi respond to their environment is paramount to developing effective mitigation strategies against disease. Carbon catabolite repression (CCR) is a global regulatory mechanism found in a wide range of microbial organisms that ensures the preferential utilization of glucose over less favourable carbon sources, but little is known about the components of CCR in filamentous fungi. Here we report three new mediators of CCR in the devastating rice blast fungus Magnaporthe oryzae: the sugar sensor Tps1, the Nmr1-3 inhibitor proteins, and the multidrug and toxin extrusion (MATE)-family pump, Mdt1. Using simple plate tests coupled with transcriptional analysis, we show that Tps1, in response to glucose-6-phosphate sensing, triggers CCR via the inactivation of Nmr1-3. In addition, by dissecting the CCR pathway using Agrobacterium tumefaciens-mediated mutagenesis, we also show that Mdt1 is an additional and previously unknown regulator of glucose metabolism. Mdt1 regulates glucose assimilation downstream of Tps1 and is necessary for nutrient utilization, sporulation, and pathogenicity. This is the first functional characterization of a MATE-family protein in filamentous f...Continue Reading

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Citations

Jul 9, 2014·Annual Review of Phytopathology·Christian P KubicekN Louise Glass
May 23, 2014·Annual Review of Phytopathology·Jessie FernandezRichard A Wilson
Jun 3, 2014·Fungal Genetics and Biology : FG & B·Yi XiongN Louise Glass
Aug 10, 2013·Scientific Reports·Jessie FernandezRichard A Wilson
Aug 8, 2014·Molecular Microbiology·Jessie FernandezRichard A Wilson
Jan 13, 2016·Environmental Microbiology·Anil KumarAsis Datta
May 6, 2015·Molecular Genetics and Genomics : MGG·Dianguang XiongChengming Tian
Mar 12, 2015·BMC Genomics·Agustina LlanosJean-Luc Parrou
Apr 19, 2017·Nature Microbiology·Margarita Marroquin-GuzmanRichard A Wilson
Dec 3, 2016·Journal of Hazardous Materials·Bin WangShihua Wang
Jan 4, 2018·International Journal of Molecular Sciences·Muhammad AdnanGuodong Lu
Feb 9, 2018·Molecular Plant-microbe Interactions : MPMI·Jasmine PhamEdgar Huitema
May 26, 2018·G3 : Genes - Genomes - Genetics·Laure Nicolas Annick RiesGustavo H Goldman
Apr 2, 2019·Molecular Plant-microbe Interactions : MPMI·Yuxin YanJianping Lu
Sep 14, 2019·Microbiology·Richard A WilsonJanet D Wright
Dec 30, 2014·Journal of Experimental Botany·Joan BarauGonçalo Amarante Guimarães Pereira

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Methods Mentioned

BETA
NMR
PCR
two-hybrid
Co-IP
pull-down
protein assay

Software Mentioned

TMpred
Scaffold
PSORTII
MASCOT
Proteome
PSIPRED
netprimer

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