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Principles of cell-free genetic circuit assembly

Proceedings of the National Academy of Sciences of the United States of America

Oct 28, 2003

Vincent NoireauxAlbert Libchaber

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Abstract

Cell-free genetic circuit elements were constructed in a transcription-translation extract. We engineered transcriptional activation and repression cascades, in which the protein product of each stage is the input required to drive or block the following stage. Although we can find regi...read more

Mentioned in this Paper

Luciferases
Protein Biosynthesis
Genetic Techniques
Cell-Free System
Poly(A) Tail
Green Fluorescent Proteins
Genetic Markers
Luminescent Proteins
Transcriptional Activation
Episomes
263
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Principles of cell-free genetic circuit assembly

Proceedings of the National Academy of Sciences of the United States of America

Oct 28, 2003

Vincent NoireauxAlbert Libchaber

PMID: 14559971

DOI: 10.1073/pnas.2135496100

Abstract

Cell-free genetic circuit elements were constructed in a transcription-translation extract. We engineered transcriptional activation and repression cascades, in which the protein product of each stage is the input required to drive or block the following stage. Although we can find regi...read more

Mentioned in this Paper

Luciferases
Protein Biosynthesis
Genetic Techniques
Cell-Free System
Poly(A) Tail
263

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