Oct 16, 2003

Principles of cell-free genetic circuit assembly

Proceedings of the National Academy of Sciences of the United States of America
Vincent NoireauxAlbert Libchaber


Cell-free genetic circuit elements were constructed in a transcription-translation extract. We engineered transcriptional activation and repression cascades, in which the protein product of each stage is the input required to drive or block the following stage. Although we can find regions of linear response for single stages, cascading to subsequent stages requires working in nonlinear regimes. Substantial time delays and dramatic decreases in output production are incurred with each additional stage because of a bottleneck at the translation machinery. Faster turnover of RNA message can relieve competition between genes and stabilize output against variations in input and parameters.

  • References22
  • Citations84
  • References22
  • Citations84


Mentioned in this Paper

Alkalescens-Dispar Group
Transcription, Genetic
Genetic Markers
Protein Biosynthesis
Green Fluorescent Proteins
Biochemical Turnover
Poly(A) Tail
Luminescent Proteins
Cell-Free System
Genetic Techniques

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