Principles of designing interpretable optogenetic behavior experiments

Learning & Memory
Brian D AllenEdward S Boyden

Abstract

Over the last decade, there has been much excitement about the use of optogenetic tools to test whether specific cells, regions, and projection pathways are necessary or sufficient for initiating, sustaining, or altering behavior. However, the use of such tools can result in side effects that can complicate experimental design or interpretation. The presence of optogenetic proteins in cells, the effects of heat and light, and the activity of specific ions conducted by optogenetic proteins can result in cellular side effects. At the network level, activation or silencing of defined neural populations can alter the physiology of local or distant circuits, sometimes in undesired ways. We discuss how, in order to design interpretable behavioral experiments using optogenetics, one can understand, and control for, these potential confounds.

References

Jan 1, 1987·Lasers in Surgery and Medicine·Z EliasJ T Brown
Sep 27, 1994·Proceedings of the National Academy of Sciences of the United States of America·A HoffmannG Büldt
Nov 3, 1994·Nature·T OkanoY Fukada
Jul 15, 1999·Biochemical and Biophysical Research Communications·H S LiuN J Ke
Oct 6, 1999·Current Opinion in Biotechnology·D Metzger, R Feil
Jun 20, 2001·Nature Reviews. Molecular Cell Biology·M J BerridgeM D Bootman
Jan 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Shaowen BaoRichard F Thompson
Nov 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Georg NagelErnst Bamberg
Dec 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Tanjew DittgenPavel Osten
Jan 28, 2005·Learning & Behavior·Bill P Godsil, Michael S Fanselow
Mar 15, 2005·Journal of Neuroscience Methods·Xiaoxi ZhuangRené Hen
Aug 24, 2005·Nature Neuroscience·Edward S BoydenKarl Deisseroth
Sep 1, 2005·Neuron·Tara C ThiagarajanRichard W Tsien
Dec 6, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Ronald L KleinRichard M Zweig
Apr 6, 2007·Nature·Feng ZhangKarl Deisseroth
Sep 15, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Shiaoching GongCharles R Gerfen
Dec 28, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Viviana GradinaruKarl Deisseroth
Mar 12, 2008·Biochemical and Biophysical Research Communications·D ZimmermannV L Sukhorukov
Jul 11, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Deniz AtasoyScott M Sternson
Jul 16, 2008·Nature Neuroscience·Nicolas ToniAlejandro F Schinder
Aug 5, 2008·Brain Cell Biology·Viviana GradinaruKarl Deisseroth
Aug 9, 2008·PLoS Biology·Stacey L EdwardsKenneth G Miller
Oct 22, 2008·Brain Cell Biology·Shengli ZhaoGuoping Feng
Dec 17, 2008·Nature Neuroscience·André BerndtKarl Deisseroth
Apr 28, 2009·Nature·Vikaas S SohalKarl Deisseroth
Sep 29, 2009·Current Opinion in Neurobiology·Herwig Baier, Ethan K Scott
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Aristides B ArrenbergHerwig Baier

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Citations

Dec 25, 2015·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Michelle Y ChengGary K Steinberg
Aug 5, 2015·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Benjamin T SaundersPatricia H Janak
Jun 28, 2015·Neuroscience·L M YagerS M Ferguson
Oct 11, 2016·ELife·Elizabeth Ak Phillips, Andrea R Hasenstaub
Feb 16, 2018·Expert Opinion on Drug Discovery·Michał KiełbusAdolfo Rivero-Müller
Jan 10, 2018·Frontiers in Neuroscience·Jean DelbekeDimiter Prodanov
Jul 21, 2016·Nature Communications·Mercè Izquierdo-SerraPau Gorostiza
Apr 1, 2020·EMBO Molecular Medicine·Alexander DieterTobias Moser
Jan 2, 2017·Microsystems & Nanoengineering·John P SeymourEuisik Yoon
Jun 19, 2019·Nature Neuroscience·Scott F OwenAnatol C Kreitzer
Sep 19, 2019·Journal of Neurophysiology·Xiankai MengAlbert S B Edge
Jan 9, 2020·Nature Reviews. Neuroscience·Katie A Ferguson, Jessica A Cardin
Jan 29, 2019·Frontiers in Systems Neuroscience·Sarah A JohnsonSara N Burke
May 12, 2015·Médecine sciences : M/S·Marie VandecasteeleGuillaume P Dugué
Apr 14, 2017·Scientific Reports·John H StockleyRagnhildur T Káradóttir
Jul 6, 2017·Current Protocols in Neuroscience·Alexander Z HarrisEkaterina Likhtik
May 26, 2018·Science Advances·Alex SavchenkoElena Molokanova
Nov 20, 2018·Frontiers in Neuroscience·Marcela Garita-HernandezDeniz Dalkara
Aug 4, 2018·Nature Protocols·Annabelle C SingerLi-Huei Tsai
Feb 11, 2020·Frontiers in Neuroinformatics·Francesca MandinoJoanes Grandjean
Jul 1, 2020·Frontiers in Physiology·Yoko B WangAmanda J Page
Dec 17, 2016·Neurophotonics·Gonzalo Arias-GilMichael Thomas Lippert
Jun 11, 2020·Cell Reports·Nicole A RepinaDavid V Schaffer
Nov 4, 2020·Proceedings of the National Academy of Sciences of the United States of America·Andrea BariSusumu Tonegawa
Nov 29, 2020·Biochimica Et Biophysica Acta. Bioenergetics·Masaki TsujimuraHiroshi Ishikita
Feb 8, 2018·Current Opinion in Neurobiology·Steffen Be Wolff, Bence P Ölveczky
Mar 23, 2021·Journal of Neural Engineering·Valentina PaggiStéphanie P Lacour
May 22, 2021·Advanced Science·Almudena Barbero-CastilloPau Gorostiza
Jul 24, 2021·Nature Communications·Takanori MatsubaraTakayuki Yamashita
Aug 26, 2021·Cell Reports·Justin N SiemianYeka Aponte

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