Printed paper sensors for serum lactate dehydrogenase using pullulan-based inks to immobilize reagents

Analytical Chemistry
Balamurali KannanJohn D Brennan

Abstract

In this study, a paper-based point-of-care (POC) colorimetric biosensor was developed for the detection of lactate dehydrogenase in serum using a nonporous, oxygen impermeable reversibly gelling polysaccharide material based on pullulan. The pullulan could be printed onto paper surfaces along with all required assay reagents, providing a means for high-stability immobilization of all reagents on paper. Serum containing lactate dehydrogenase (LDH) was directly spotted on to the pullulan-coated bioactive paper and provided quantitative colorimetric data that was comparable to that obtained with a conventional plate-reader method. The paper strip was found to be highly stable and could be stored at 4 °C for at least 10 weeks with no loss in performance, as compared to a complete loss in performance within 1 day when the reagents were printed without the stabilizing polysaccharide. The ease of fabrication coupled with the high stability of the printed reagents provides a facile platform for easily manufactured POC sensors.

References

Jul 1, 1980·The Journal of Pediatrics·J J QuinnC N Frantz
Sep 1, 1994·Journal of Clinical Gastroenterology·W M Cassidy, T B Reynolds
Jan 11, 2007·Angewandte Chemie·Andres W MartinezGeorge M Whitesides
Dec 17, 2009·Analytical Chemistry·Andres W MartinezEmanuel Carrilho
Oct 28, 2011·Analytical Chemistry·S M Zakir Hossain, John D Brennan
Jan 6, 2012·Journal of Agricultural and Food Chemistry·Stefano FarrisLuciano Piergiovanni
Apr 25, 2012·Analytical and Bioanalytical Chemistry·S M Zakir HossainJohn D Brennan
Oct 20, 2012·Lab on a Chip·Sana Jahanshahi-AnbuhiCarlos D M Filipe
Feb 12, 2013·Methods in Molecular Biology·Francis Ka-Ming ChanMaría José De Rosa
May 9, 2013·Lab on a Chip·Ali Kemal YetisenChristopher R Lowe
Apr 26, 2014·Angewandte Chemie·Sana Jahanshahi-AnbuhiCarlos D M Filipe

❮ Previous
Next ❯

Citations

Feb 20, 2016·Analytical Chemistry·Fatemeh DerikvandHarry Brumer
Sep 17, 2016·Critical Reviews in Analytical Chemistry·Michael J KangasAndrea E Holmes
Dec 13, 2016·Analytical Chemistry·Yuanyuan YangCharles S Henry
Jan 17, 2017·Chembiochem : a European Journal of Chemical Biology·Ping-Yao HsiehYingfu Li
Feb 16, 2017·Lab on a Chip·Sana Jahanshahi-AnbuhiCarlos D M Filipe
Dec 7, 2018·Chembiochem : a European Journal of Chemical Biology·M Monsur AliWeian Zhao
Jan 17, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Rudi LiuYingfu Li
Jul 13, 2018·Frontiers in Chemistry·Alexander BöhmMarkus Biesalski
Sep 28, 2017·Scientific Reports·M Monsur AliJohn D Brennan
Mar 1, 2016·Chemical Science·Sana Jahanshahi-AnbuhiCarlos D M Filipe
Jun 28, 2019·Scientific Reports·Cecilia Pegelow HalvorsenAman Russom
Jul 14, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Bhaskar Manna, C Retna Raj
Sep 13, 2020·Biosensors·Emiliano Martínez-PeriñánEncarnación Lorenzo
Jan 20, 2021·Analytical Methods : Advancing Methods and Applications·Lael WentlandElain Fu
Mar 31, 2021·Applied Biochemistry and Biotechnology·Jian-Hui LiYun-Peng Bai
Jul 7, 2021·Chemical Society Reviews·Erin M McConnellYingfu Li
Dec 29, 2016·Analytical Chemistry·Jungmi LeeJoon Myong Song
Mar 15, 2019·Analytical Chemistry·Michael G WolfeJohn D Brennan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biosensor Development & Applications

Biosensor development and applications describe recent progress in biosensor development and their applications in the field of antibiotics and metabolic engineering. Discover the latest research on biosensor development and applications in this feed.

Related Papers

Clinica Chimica Acta; International Journal of Clinical Chemistry
J F Burd, M Usategui-Gomez
Monatsschrift Kinderheilkunde : Organ der Deutschen Gesellschaft für Kinderheilkunde
M OrtM Teplý
© 2021 Meta ULC. All rights reserved