PMID: 9003061Feb 1, 1997Paper

Prion protein fragment 106-126 differentially induces heme oxygenase-1 mRNA in cultured neurons and astroglial cells

Journal of Neurochemistry
M RizzardiniL Cantoni

Abstract

Heme oxygenase (HO), which catalyzes the degradation of heme, has two isozymes (HO-1 and HO-2). In brain the noninducible HO-2 isoform is predominant, whereas the inducible HO-1 is a marker of oxidative stress. Because brain oxidative stress might be present in prion-related encephalopathies (PREs), as in other neurodegenerative diseases, we investigated whether HO-1 mRNA was induced in neuronal and astroglial cell cultures by a peptide corresponding to residue 106-126 of human prion protein (PrP). This peptide is amyloidogenic, and when added in vitro to cultured cells it reproduces the neuronal death and astroglial proliferation and hypertrophy occurring in PREs. HO-1 mRNA did not accumulate in rat cultured neurons from hippocampus or cortex exposed to PrP 106-126 (50 microM for 5 days). PrP 106-126 induced HO-1 mRNA accumulation in rat astroglial cultures depending on the exposure time and concentration, being maximal (33-fold) after 7 days of exposure at 50 microM. The nonamyloidogenic amidated or amidated-acetylated PrP 106-126 was ineffective, as was a scrambled peptide used as control. N-Acetylcysteine reduced (50%) the accumulation of HO-1 mRNA in astroglial cells after PrP 106-126 (25 microM) given for 5 days. Thus, ox...Continue Reading

Citations

Dec 31, 2019·Oxidative Medicine and Cellular Longevity·ShiDa ChenJulia Li Zhong
Nov 27, 1999·The Journal of Biological Chemistry·S CapellariR B Petersen
Jul 19, 2002·Journal of Neurochemistry·David R Brown
Sep 17, 2002·Antioxidants & Redox Signaling·Shigeki ShibaharaKazuhiro Takahashi
Feb 18, 2006·Cell and Tissue Research·W M AmselgruberM Büttner
Jul 17, 1998·Neurobiology of Disease·D R BrownH A Kretzschmar
May 30, 1998·Experimental Neurology·B E DwyerR N Nishimura
May 26, 2007·Journal of Neuroimmunology·Jae-Il KimYong-Sun Kim

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