PRISEs (progesterone 5β-reductase and/or iridoid synthase-like 1,4-enone reductases): Catalytic and substrate promiscuity allows for realization of multiple pathways in plant metabolism

Phytochemistry
Karin SchmidtWolfgang Kreis

Abstract

PRISEs (progesterone 5β-reductase and/or iridoid synthase-like 1,4-enone reductases) are involved in cardenolide and iridoid biosynthesis. We here investigated a PRISE (rAtSt5βR) from Arabidopsis thaliana, a plant producing neither cardenolides nor iridoids. The structure of rAtSt5βR was elucidated with X-ray crystallography and compared to the known structures of PRISEs from Catharanthus roseus (rCrISY) and Digitalis lanata (rDlP5βR). The three enzymes show a high degree of sequence and structure conservation in the active site. Amino acids previously considered to allow discrimination between progesterone 5β-reductase and iridoid synthase were interchanged among rAtSt5βR, rCrISY and rDlP5βR applying site-directed mutagenesis. Structural homologous substitutions had different effects, and changes in progesterone 5β-reductase and iridoid synthase activity were not correlated in all cases. Our results help to explain fortuitous emergence of metabolic pathways and product accumulation. The fact that PRISEs are found ubiquitously in spermatophytes insinuates that PRISEs might have a more general function in plant metabolism such as, for example, the detoxification of reactive carbonyl species.

References

Jun 19, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R D'Ari, J Casadesús
Feb 16, 2002·Protein Science : a Publication of the Protein Society·Yvonne KallbergBengt Persson
Mar 28, 2002·Plant & Cell Physiology·Ji Hyung JunHong Gil Nam
Apr 16, 2003·The Plant Journal : for Cell and Molecular Biology·Emmanuelle AlmérasEdward E Farmer
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·E Krissinel, K Henrick
Mar 24, 2007·Trends in Biotechnology·Karl Hult, Per Berglund
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Aug 8, 2007·Journal of Molecular Biology·Evgeny Krissinel, Kim Henrick
Aug 1, 2007·Journal of Applied Crystallography·Airlie J McCoyRandy J Read
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
Mar 20, 2010·Annual Review of Biochemistry·Olga Khersonsky, Dan S Tawfik
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·P EmsleyK Cowtan
May 5, 2010·Nucleic Acids Research·Mickael GoujonRodrigo Lopez
Aug 6, 2011·PloS One·Rosa TarríoFrancisco Rodríguez-Trelles
Jul 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Richard A NotebaartBalázs Papp
Jan 13, 2015·Trends in Biochemical Sciences·Shelley D Copley
Jan 13, 2015·Molecular Plant·Jennifer MunkertAlain Goossens
Nov 10, 2015·Nature Chemical Biology·Hajo KriesSarah E O'Connor
Jul 14, 2017·The Journal of Biological Chemistry·Hajo KriesSarah E O'Connor
Aug 23, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Beibei XiangYong Wang

❮ Previous
Next ❯

Citations

Feb 5, 2019·Journal of Experimental Botany·Wolfgang Kreis, Jennifer Munkert
Jul 10, 2020·BMC Plant Biology·C V FilippiV V Lia
May 29, 2020·Metabolic Engineering·Quentin M DudleyMichael C Jewett

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.