Prism-based multicolor fluorescence correlation spectrometer

Optics Letters
Ling Chin HwangThorsten Wohland

Abstract

We report the design and application of a prism-based detection system for fluorescence (cross) correlation spectroscopy. The system utilizes a single laser wavelength for the simultaneous excitation of several dyes of different emission spectra. Fluorescence light is spectrally separated with a prismatic setup, and wavelengths are selected by scanning a fiber-coupled avalanche photodiode across the image spots. Multicolor autocorrelations are demonstrated with standard and tandem dyes, and fluorescence cross-correlation measurements of biotinylated nanocontainers and streptavidin are presented. This spectrometer offers high optical stability and no focal volume mismatch for the multicolor detection of molecular dynamics and interactions, with single-molecule sensitivity.

References

Aug 10, 2000·Proceedings of the National Academy of Sciences of the United States of America·T D LacosteS Weiss
Dec 5, 2002·Current Opinion in Structural Biology·Nancy L ThompsonNoah W Allen
May 14, 2004·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Ling Chin Hwang, Thorsten Wohland

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Citations

May 5, 2007·Annual Review of Biophysics and Biomolecular Structure·Elke Haustein, Petra Schwille
Sep 18, 2007·Cell Biochemistry and Biophysics·Ling Chin Hwang, Thorsten Wohland
Nov 6, 2015·Nature Protocols·Jan W KriegerThorsten Wohland
Mar 15, 2012·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Jonas Ries, Petra Schwille
Dec 18, 2010·Optics Express·Felix BestvaterMalte Wachsmuth
Jul 1, 2018·Journal of Biomedical Optics·Jun LiaoGuoan Zheng
May 23, 2008·Analytical Chemistry·Mark LowryIsiah M Warner

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