PMID: 9006024Feb 1, 1997Paper

Pristinamycin I biosynthesis in Streptomyces pristinaespiralis: molecular characterization of the first two structural peptide synthetase genes

Journal of Bacteriology
V de Crécy-LagardD Thibaut

Abstract

Two genes involved in the biosynthesis of the depsipeptide antibiotics pristinamycins I (PI) produced by Streptomyces pristinaespiralis were cloned and sequenced. The 1.7-kb snbA gene encodes a 3-hydroxypicolinic acid:AMP ligase, and the 7.7-kb snbC gene encodes PI synthetase 2, responsible for incorporating L-threonine and L-aminobutyric acid in the PI macrocycle. snbA and snbC, which encode the two first structural enzymes of PI synthesis, are not contiguous. Both genes are located in PI-specific transcriptional units, as disruption of one gene or the other led to PI-deficient strains producing normal levels of the polyunsaturated macrolactone antibiotic pristinamycin II, also produced by S. pristinaespiralis. Analysis of the deduced amino acid sequences showed that the SnbA protein is a member of the adenylate-forming enzyme superfamily and that the SnbC protein contains two amino acid-incorporating modules and a C-terminal epimerization domain. A model for the initiation of PI synthesis analogous to the established model of initiation of fatty acid synthesis is proposed.

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Citations

Aug 22, 2007·Journal of Biomedicine & Biotechnology·Samiha SioudLotfi Mellouli
Sep 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Gregory L Challis, David A Hopwood
Nov 8, 2008·Molecular Microbiology·Zoe HeatherAndrew S Waller
Dec 1, 2008·Indian Journal of Microbiology·Renu SolankiRup Lal
Apr 22, 2015·Chembiochem : a European Journal of Chemical Biology·Carolina Cano-PrietoCarlos Olano
Nov 9, 2016·Frontiers in Pharmacology·Shivankar AgrawalSunil K Deshmukh
Mar 10, 2006·Microbiology and Molecular Biology Reviews : MMBR·Jan Grünewald, Mohamed A Marahiel
Apr 18, 2003·Journal of Bacteriology·David F AckerleyIain L Lamont
Mar 10, 2004·Applied and Environmental Microbiology·Daisuke IshiyamaJulian Davies
Aug 5, 2000·Applied and Environmental Microbiology·R H Ffrench-ConstantF R Blattner
Apr 19, 2000·Antimicrobial Agents and Chemotherapy·M SteffenskyL Heide
Mar 27, 1999·Journal of Bacteriology·B Nowak-ThompsonJ E Loper
Feb 11, 2005·Chemical Reviews·Tariq A Mukhtar, Gerard D Wright
Feb 11, 2005·Chemical Reviews·Stephan A Sieber, Mohamed A Marahiel
Jul 17, 1998·Current Opinion in Chemical Biology·C T WalshR S Flugel
Jul 17, 1998·Current Opinion in Chemical Biology·H D Mootz, M A Marahiel
Feb 18, 1999·Chemistry & Biology·D Konz, M A Marahiel
Nov 24, 1999·Infection and Immunity·A M WertheimerJ H Crosa
Feb 1, 1997·Journal of Bacteriology·D ThibautF Blanche
Aug 1, 1997·Chemistry & Biology·M A Marahiel

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