Pristine environments harbor a new group of oligotrophic 2,4-dichlorophenoxyacetic acid-degrading bacteria.

Applied and Environmental Microbiology
Y KamagataJ M Tiedje

Abstract

2,4-Dichlorophenoxyacetic acid (2,4-D)-degrading bacteria were isolated from pristine environments which had no history of 2,4-D exposure. By using 2,4-D dye indicator medium or 14C-labeled 2,4-D medium, six strains were isolated from eight enrichment cultures capable of degrading 2,4-D. Phylogenetic analyses based on 16S ribosomal DNA (rDNA) sequencing and physiological properties revealed that one isolate from Hawaiian volcanic soil could be classified in the genus Variovorax (a member of the beta subdivision of the class Proteobacteria) and that the other five isolates from Hawaiian volcanic soils, Saskatchewan forest soil, and Chilean forest soil have 16S rDNAs with high degrees of similarity to those of the Bradyrhizobium group (a member of the alpha subdivision of the class Proteobacteria). All the isolates grow slowly on either nutrient media (0.1 x Bacto Peptone-tryptone-yeast extract-glucose [PTYG] or 0.1 x Luria broth [LB] medium) or 2,4-D medium, with mean generation times of 16 to 30 h, which are significantly slower than previously known 2,4-D degraders. Nutrient-rich media such as full-strength PTYG and LB medium did not allow their growth. PCR amplification using internal consensus sequences of tfdA (a gene encod...Continue Reading

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Citations

Nov 9, 2006·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Vasili M TravkinLudmila A Golovleva
Nov 10, 2012·Applied Microbiology and Biotechnology·Om PrakashJoel E Kostka
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Apr 9, 2015·World Journal of Microbiology & Biotechnology·Lizhen HanDegang Zhao
Feb 3, 2005·Archives of Microbiology·Monika ThielMichael Schlömann
May 15, 2012·Applied and Environmental Microbiology·Marek StibalCarsten S Jacobsen
Nov 30, 2012·Applied Microbiology and Biotechnology·Barbara SatolaAlexander Steinbüchel
Aug 21, 2015·Environmental Toxicology and Chemistry·Tadeusz PaszkoIzabella Jackowska
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Aug 6, 2004·Applied and Environmental Microbiology·Liz J Shaw, Richard G Burns
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Feb 8, 2006·Applied and Environmental Microbiology·Jacob BaelumCarsten Suhr Jacobsen
Jun 6, 2014·World Journal of Microbiology & Biotechnology·Lizhen HanDegang Zhao
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Oct 1, 2019·Microbial Biotechnology·Panu PimviriyakulPimchai Chaiyen
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