Prm1 functions as a disulfide-linked complex in yeast mating.

The Journal of Biological Chemistry
Valerie N Olmo, Eric Grote

Abstract

Prm1 is a pheromone-induced membrane glycoprotein that promotes plasma membrane fusion in yeast mating pairs. HA-Prm1 migrates at twice its expected molecular weight on non-reducing SDS-PAGE gels and coprecipitates with Prm1-TAP, indicating that Prm1 is a disulfide-linked homodimer. The N terminus of a plasma membrane-localized GFP-Prm1 endocytic mutant projects into the cytoplasm, where it is protected from low pH quenching in live cells and from external protease in spheroplasts. In a revised topological map, Prm1 has four transmembrane domains and two large extracellular loops. Mutation of all four cysteines in the extracellular loops blocked disulfide bond formation and destabilized the Prm1 homodimer without preventing Prm1 transport to contact sites in mating pairs. Cys(120) in loop 1 and Cys(545) in loop 2 form disulfide cross-links in the Prm1 homodimer and are required for fusion activity. Cys(120) lies between a hydrophobic segment formerly thought to be a transmembrane domain and an amphipathic helix. An interaction between either of these regions and the opposing membrane could promote fusion.

References

May 1, 1980·Journal of Bacteriology·J H Scott, R Schekman
Nov 1, 1994·The Journal of Cell Biology·E C GaynorS D Emr
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·H J RyserG J DiSciullo
Mar 14, 1998·The Journal of Cell Biology·S ErdmanM Snyder
Jul 11, 2000·Molecular Biology of the Cell·E Leikina, L V Chernomordik
Jun 14, 2001·Methods : a Companion to Methods in Enzymology·O PuigB Séraphin
Jun 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·Y Hua, R H Scheller
Jul 25, 2003·The Journal of Biological Chemistry·Eric M MullerKyle W Cunningham
Dec 14, 2004·Eukaryotic Cell·Hui JinEric Grote
Apr 16, 2005·Science·Elizabeth H Chen, Eric N Olson
May 10, 2005·The Biochemical Journal·Colin RickmanBazbek Davletov
May 25, 2005·The Journal of Cell Biology·Christoph JüschkeMatthias Seedorf
Feb 24, 2006·Molecular Biology of the Cell·Scott NolanEric Grote
Jun 2, 2006·Molecular Biology of the Cell·Nan-Nan ZhangKyle W Cunningham
Dec 8, 2006·Molecular Biology of the Cell·Pablo S AguilarPeter Walter
May 10, 2007·Developmental Cell·Amir SapirBenjamin Podbilewicz
Jun 15, 2007·Antioxidants & Redox Signaling·Emmanuel FenouilletIan M Jones
Oct 2, 2007·Nature Structural & Molecular Biology·Feng LiDavid Tareste
Feb 27, 2008·The Journal of Cell Biology·Hui JinEric Grote
Jun 17, 2008·Nature Structural & Molecular Biology·Xiaobing LuYeon-Kyun Shin
Nov 4, 2008·Methods in Molecular Biology·Casey A Ydenberg, Mark D Rose

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Citations

Mar 5, 2013·Trends in Genetics : TIG·Pablo S AguilarMelissa Wong
Mar 8, 2013·Open Biology·Laura MerliniSophie G Martin
Apr 27, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Meital Oren-Suissa, Benjamin Podbilewicz
Jun 4, 2019·Journal of Cell Science·Sophie G Martin
Sep 1, 2021·Molecular Microbiology·Carla E LanzeJames B Konopka

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