PRMT7 contributes to the metastasis phenotype in human non-small-cell lung cancer cells possibly through the interaction with HSPA5 and EEF2

OncoTargets and Therapy
Dezhi ChengPeng Zhang

Abstract

Non-small-cell lung cancer (NSCLC) constitutes the leading cause of cancer death in humans. Previous studies revealed the essential role of the protein arginine methyltransferase 7 (PRMT7) in promoting metastasis in breast cancer. However, its function and potential mechanism in NSCLC remain unclear. The gene expression of PRMT7 between lung cancer tissues and normal tissues was studied with online database (http://medicalgenome.kribb.re.kr/GENT/). NSCLC cell lines with specific gene overexpression were constructed with lentivirus transduction. Matrigel invasion and colony formation assays were performed to evaluate the invasion and colony formation abilities. Co-immunoprecipitation coupled with mass spectrometry analysis was performed to explore the potential interaction proteins of PRMT7. Bioinformatic analysis was performed with Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases. Online analysis of gene expression patterns revealed the relatively high expression of PRMT7 in lung cancer tissues. PRMT7 overexpression was able to promote the invasion and colony formation of A549 and SPC-A1 cells. A total of 19 in-common proteins shared by both NSCLC cell lines were identified to be interacting with PRMT7 and fo...Continue Reading

Citations

Jan 29, 2020·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Fangqing LiXueping Zhong
Jan 10, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Junji ZhuWuhan Xiao
Dec 25, 2019·Signal Transduction and Targeted Therapy·Yuan ChengXiawei Wei
Dec 17, 2020·Signal Transduction and Targeted Therapy·Yuan ChengXiawei Wei
Mar 21, 2021·Nature Reviews. Drug Discovery·Qin WuDalia Barsyte-Lovejoy
May 20, 2021·Experimental & Molecular Medicine·Jee Won HwangYong Kee Kim
May 28, 2021·Acta Biochimica Et Biophysica Sinica·Wuwen ZhangGuoqing Tong
Aug 28, 2021·Life·Levon Halabelian, Dalia Barsyte-Lovejoy

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Methods Mentioned

BETA
immunoprecipitation
electrophoresis
co-immunoprecipitation
xenograft
protein folding

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