PRMT9 is a type II methyltransferase that methylates the splicing factor SAP145

Nature Communications
Yanzhong YangMark T Bedford

Abstract

The human genome encodes a family of nine protein arginine methyltransferases (PRMT1-9), whose members can catalyse three distinct types of methylation on arginine residues. Here we identify two spliceosome-associated proteins-SAP145 and SAP49-as PRMT9-binding partners, linking PRMT9 to U2 snRNP maturation. We show that SAP145 is methylated by PRMT9 at arginine 508, which takes the form of monomethylated arginine (MMA) and symmetrically dimethylated arginine (SDMA). PRMT9 thus joins PRMT5 as the only mammalian enzymes capable of depositing the SDMA mark. Methylation of SAP145 on Arg 508 generates a binding site for the Tudor domain of the Survival of Motor Neuron (SMN) protein, and RNA-seq analysis reveals gross splicing changes when PRMT9 levels are attenuated. These results identify PRMT9 as a nonhistone methyltransferase that primes the U2 snRNP for interaction with SMN.

References

Feb 7, 1977·Biochemical and Biophysical Research Communications·L C BoffaV G Allfrey
May 1, 1995·Molecular and Cellular Biology·Q Liu, G Dreyfuss
Sep 30, 1998·Progress in Nucleic Acid Research and Molecular Biology·J D Gary, S Clarke
Jan 5, 2002·The Journal of Biological Chemistry·Westley J FriesenGideon Dreyfuss
Aug 2, 2003·Methods : a Companion to Methods in Enzymology·Kenichi Nishioka, Danny Reinberg
Oct 12, 2004·The Biochemical Journal·Rafal SwierczMark T Bedford
Jun 10, 2005·The Journal of Biological Chemistry·Naganari OhkuraToshihiko Tsukada
Jul 30, 2005·The Journal of Biological Chemistry·Jaeho LeeMark T Bedford
Feb 21, 2006·Biochemical and Biophysical Research Communications·Jeffry R CookSidney Pestka
Apr 19, 2007·The Journal of Biological Chemistry·Rafal SwierczMark T Bedford
Jan 20, 2009·Molecular Cell·Mark T Bedford, Steven G Clarke
Jan 6, 2010·Nucleic Acids Research·Matthew J HarrisonDennis H Dowhan
Oct 20, 2010·Proceedings of the National Academy of Sciences of the United States of America·Xian DengXiaofeng Cao
Nov 16, 2010·FEBS Letters·Alessandra Di Lorenzo, Mark T Bedford
Sep 15, 2011·Nature Reviews. Molecular Cell Biology·Chen ChenTony Pawson
Jan 14, 2012·The Journal of Biological Chemistry·Cecilia I Zurita-LopezSteven G Clarke
Aug 7, 2012·Molecular & Cellular Proteomics : MCP·Thomas UhlmannOreste Acuto
Dec 14, 2012·Nature Reviews. Cancer·Yanzhong Yang, Mark T Bedford
Apr 16, 2013·Chembiochem : a European Journal of Chemical Biology·Hao ZengWei Xu
Oct 17, 2013·Molecular & Cellular Proteomics : MCP·Ailan GuoMichael J Comb
Jan 24, 2014·Nature Reviews. Molecular Cell Biology·A Gregory Matera, Zefeng Wang
Mar 4, 2014·Biochimica Et Biophysica Acta·Sitaram Gayatri, Mark T Bedford

❮ Previous
Next ❯

Citations

Feb 9, 2016·Cell Reports·Roméo Sébastien BlancStéphane Richard
Nov 13, 2015·Journal of Chemical Information and Modeling·Ran ZhouCheng Luo
Nov 28, 2015·Archives of Biochemistry and Biophysics·Yalemi MoralesJoan M Hevel
Jan 21, 2016·Expert Opinion on Investigational Drugs·Hao HuY George Zheng
Feb 10, 2016·Proceedings of the National Academy of Sciences of the United States of America·Erik W DeblerPete Stavropoulos
Dec 22, 2015·ACS Chemical Biology·Jakob Fuhrmann, Paul R Thompson
Apr 5, 2016·Mediators of Inflammation·Ji Hye KimJae Youl Cho
Aug 26, 2015·Cellular and Molecular Life Sciences : CMLS·Hunain AlamMin Gyu Lee
May 3, 2015·Biochemical and Biophysical Research Communications·Chul-Gon YeomSoo-Hyun Park
Oct 28, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Khondker Ayesha AkterTakeshi Senga
Feb 7, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Jianqiang Bao, Mark T Bedford
Jan 4, 2017·Expert Review of Proteomics·Chao Peng, Catherine Cl Wong
Mar 25, 2017·Chemical Reviews·H Ümit KaniskanJian Jin
Apr 10, 2017·FEBS Letters·Virginie F RheinJohn E Walker
Aug 16, 2016·Chemistry : a European Journal·Astrid KnuhtsenDaniel Sejer Pedersen
Oct 5, 2017·Nucleic Acids Research·Madhumitha RengasamyMartin J Walsh
Jan 15, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lindsay M WebbMireia Guerau-de-Arellano
May 22, 2018·Expert Opinion on Therapeutic Targets·Emily SmithRobert A Baiocchi
Sep 7, 2017·Proceedings of the National Academy of Sciences of the United States of America·Kanishk JainSteven G Clarke
Sep 20, 2018·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Melody D FultonY George Zheng
Sep 20, 2016·Nature Communications·Corina T Madreiter-SokolowskiWolfgang F Graier
Jan 9, 2019·Journal of Molecular Endocrinology·Shu-Ching M WangGeorge E O Muscat
Jan 31, 2019·Molecular Biology of the Cell·Nasim HaghandishJocelyn Côté
Jan 1, 2015·Cell Discovery·Fengjuan LvJianping Ding
Jul 22, 2018·Nature Communications·Antonella ScaglionePatrizia Casaccia
May 28, 2019·Cellular and Molecular Life Sciences : CMLS·Sunil Kumar TewaryMeng-Chiao Ho
May 17, 2015·The Journal of Biological Chemistry·Andrea HadjikyriacouSteven G Clarke
Dec 12, 2019·Molecular Neurobiology·Alexandre Couto E SilvaHung Wen Lin
Aug 25, 2016·Oncotarget·Coralie PoulardMuriel Le Romancer
Jul 9, 2016·The Journal of Biological Chemistry·Kanishk JainSteven G Clarke
Nov 21, 2018·Life Science Alliance·Donghang ChengMark T Bedford
Mar 20, 2019·Proceedings of the National Academy of Sciences of the United States of America·Jiawen FengYuxin Yin
Jul 10, 2019·International Journal of Molecular Sciences·Melody D FultonY George Zheng
Mar 7, 2020·Cellular and Molecular Life Sciences : CMLS·Chengfu Sun

❮ Previous
Next ❯

Methods Mentioned

BETA
tandem affinity purification
pull-down
co-IP
pull down
coimmunoprecipitation
coimmunoprecipitation assay
immunoprecipitation
RNA-seq
nuclear translocation
transfection

Software Mentioned

Gonnet
TopHat
ClustalW

Related Concepts

Related Feeds

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.