Pro-angiogenic scaffold-free Bio three-dimensional conduit developed from human induced pluripotent stem cell-derived mesenchymal stem cells promotes peripheral nerve regeneration.

Scientific Reports
Sadaki MitsuzawaMakoto Ikeya

Abstract

Although autologous nerve grafting is widely accepted as the gold standard treatment for segmental nerve defects, harvesting autologous nerves is highly invasive and leads to functional loss of the ablated part. In response, artificial nerve conduits made of artificial materials have been reported, but the efficacy of the nerve regeneration still needs improvement. The purpose of this study is to investigate the efficacy and mechanism of the Bio three-dimensional (3D) conduit composed of xeno-free human induced pluripotent stem cell-derived mesenchymal stem cells (iMSCs). The 5-mm nerve gap of the sciatic nerve in immunodeficient rats was bridged with the Bio 3D conduit or silicone tube. Functional and histological recovery were assessed at 8 weeks after surgery. The regenerated nerve in the Bio 3D group was significantly superior to that in the silicone group based on morphology, kinematics, electrophysiology, and wet muscle weight. Gene expression analyses demonstrated neurotrophic and angiogenic factors. Macroscopic observation revealed neovascularization both inside and on the surface of the Bio 3D conduit. Upon their subcutaneous implantation, iMSCs could induce angiogenesis. The Bio 3D conduit fabricated from iMSCs are an...Continue Reading

References

Jan 1, 1996·British Journal of Plastic Surgery·T MurashitaS Ohashi
May 4, 2001·Plastic and Reconstructive Surgery·S E MackinnonE P Trulock
Nov 26, 2003·Experimental Neurology·Annie C LeeShelly E Sakiyama-Elbert
Nov 30, 2004·Cell Transplantation·Eduardo GámezTakehisa Matsuda
Dec 20, 2007·Cell Transplantation·Tomoyuki YamakawaTakashi Nakamura
Feb 13, 2009·Scandinavian Journal of Surgery : SJS : Official Organ for the Finnish Surgical Society and the Scandinavian Surgical Society·L B Dahlin
Feb 21, 2009·Neurosurgical Focus·Tessa Gordon
Jun 9, 2009·Cell Transplantation·Lizzie Y SantiagoKacey G Marra
Mar 24, 2010·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·I V YannasP T C So
Mar 1, 2011·Journal of Neuroscience Methods·Nithya J JesurajPhilip J Johnson
Sep 15, 2011·Journal of Tissue Engineering and Regenerative Medicine·Chien-Yuan WangShih-Chieh Hung
Oct 21, 2011·Tissue Engineering. Part B, Reviews·Juliet H A Bell, John W Haycock
Jan 24, 2012·Stem Cell Research·Sarah K WalshRajiv Midha
Jan 11, 2013·Journal of Reconstructive Microsurgery·P Konofaos, J P Ver Halen
Dec 7, 2013·The Journal of Bone and Joint Surgery. American Volume·Justin W GriffinD Nicole Deal
Apr 24, 2015·Cell Transplantation·Tsubasa YamamotoMisako Nakashima
Oct 23, 2015·Journal of Tissue Engineering and Regenerative Medicine·Natsuko KakudoKenji Kusumoto
Aug 2, 2017·The Journal of Clinical Investigation·Kyosuke HinoMakoto Ikeya
Aug 25, 2017·Stem Cells Translational Medicine·Ashley N LeberfingerIbrahim T Ozbolat
Sep 30, 2017·Stem Cells Translational Medicine·Phoebe RocheFergal J O'Brien

❮ Previous
Next ❯

Citations

Dec 4, 2020·Development, Growth & Differentiation·Taiki Nakajima, Makoto Ikeya

❮ Previous
Next ❯

Methods Mentioned

BETA
light microscopy
transmission electron microscopy
FACS
PCR
Fluorescence-activated

Software Mentioned

JMP
JMP Pro
Excel
- 3D printer
ImageJ
Bio

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.