Pro-apoptotic and anti-proliferative effects of mitofusin-2 via PI3K/Akt signaling in breast cancer cells

Oncology Letters
L I MaYueping Liu

Abstract

The mitochondrial GTPase mitofusin-2 (Mfn2) gene is a novel gene characterized as a cell proliferation inhibitor. Mfn2 has previously been reported to play a role in regulating cell proliferation, apoptosis and differentiation in a number of cell types. However, there are no studies on the effect of Mfn2 in breast cancer. In this study, we aimed to elucidate the function and mechanism of Mfn2 in breast cancer. A plasmid encoding the complete Mfn2 open reading frame (pEGFP-Mfn2) was used to infect breast cancer cells. The effect of Mfn2 on proliferation was assessed by methyl thiazolyl tetrazolium and bromodeoxyuridine incorporation analyses. Flow cytometry, immunofluorescence and western blot analyses were used to test the effects of Mfn2 on cell cycle distribution and apoptosis. Additionally, the PI3K/Akt signaling pathway was analyzed after pEGFP-Mfn2 was transfected into MCF-7 cells. The results revealed that Mfn2 suppressed the proliferation of MCF-7 cells by regulating more cells at the G0/G1 phase and decreasing proliferating cell nuclear antigen and cyclin A expression. The results also demonstrated that the PI3K/Akt signaling pathway is involved in Mfn2-regulated proliferation and apoptosis. Taken together, this indicat...Continue Reading

References

Sep 20, 2001·Seminars in Cancer Biology·S Sommer, S A Fuqua
Oct 3, 2001·Physiological Reviews·S NilssonJ A Gustafsson
Dec 25, 2002·The Journal of Cell Biology·Mariusz KarbowskiRichard J Youle
Mar 9, 2004·The Lancet Oncology·Valerie SpeirsAndrew M Hanby
Aug 24, 2004·Nature Cell Biology·Kuang-Hueih ChenJian Tang
Jun 14, 2005·Gut·M E Guicciardi, G J Gores
Feb 22, 2007·Liver International : Official Journal of the International Association for the Study of the Liver·Isabel FabregatMargarita Fernández
Sep 29, 2007·Circulation Research·Xiaomei GuoRui-Ping Xiao
Dec 21, 2007·Antioxidants & Redox Signaling·Olga Martins de Brito, Luca Scorrano
Mar 29, 2008·The Journal of Biological Chemistry·Benedikt Westermann
Apr 17, 2008·Cancer Research·Anindya Bagchi, Alea A Mills
Aug 31, 2010·Medical Oncology·Baiye JinShusen Zheng
May 7, 2013·Cancer Treatment Reviews·Kamal S SainiMartine J Piccart-Gebhart
Jun 26, 2013·International Journal of Molecular Sciences·Ge-Er ZhangJi-Ren Yu

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Citations

Jul 6, 2018·Endocrine-related Cancer·Andrea NicoliniAngelo Carpi
Mar 16, 2019·The Journal of Biological Chemistry·Andrea BraganzaSruti Shiva
Apr 14, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Karen F CarvalhoMarcos R Chiaratti
Jun 13, 2017·Frontiers in Oncology·Maria Sol Herrera-Cruz, Thomas Simmen
Dec 1, 2017·Biological & Pharmaceutical Bulletin·Ying YangYue Gao
Oct 12, 2018·Endocrine-related Cancer·Andrea NicoliniAngelo Carpi
Jul 27, 2018·Oxidative Medicine and Cellular Longevity·Ran XueJianyu Hao
Aug 29, 2018·International Journal of Molecular Sciences·Victor García-GonzálezAbril Alicia Pérez-Arias
Dec 15, 2016·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Weixin LiangXiaoyu Gu
Jan 30, 2021·Frontiers in Cell and Developmental Biology·Aurora Gil-HernándezAlejandro Silva-Palacios
Mar 16, 2021·Frontiers in Cell and Developmental Biology·Paula DíazSergio Lavandero

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