Pro-apoptotic effect of endogenous H2S on human aorta smooth muscle cells

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Guangdong YangRui Wang

Abstract

Cystathionine gamma-lyase (CSE) is a key enzyme in the trans-sulfuration pathway, which uses L-cysteine to produce hydrogen sulfide (H2S). The CSE/H2S system has been shown to play an important role in regulating cellular functions in different systems. In the present study, we overexpressed CSE in human aorta smooth muscle cells (HASMCs) using a recombinant defective adenovirus containing CSE gene (Ad-CSE). Infection of HASMCs with Ad-CSE resulted in a significant increase in the expression of CSE protein and H2S production. Ad-CSE transfection inhibited cell growth and stimulated apoptosis, as evidenced by cell viability assay, Hoechst 33258 staining, TUNEL, and caspase 3 activation. CSE-mediated apoptosis was associated with an increased ERK and p38 MAPK activation, up-regulation of p21(Cip/WAK-1), and down-regulation of cyclin D1 expression. After inhibiting endogenous background CSE gene expression, direct administration of H2S at 100 microM induced apoptosis of HASMCs. The other two endproducts of CSE-catalyzed enzymatic reaction, ammonium and pyruvate, failed to do so. These results demonstrate that overexpression of CSE stimulates SMC apoptosis due to an increased endogenous production of H2S. Adenovirus-mediated transf...Continue Reading

References

Nov 24, 1995·Science·Z XiaM E Greenberg
Jul 1, 1997·Canadian Journal of Physiology and Pharmacology·M B HershensonJ Li
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·T C HeB Vogelstein
Jul 1, 1999·Genes & Development·C J Sherr, J M Roberts
Oct 26, 2000·Nature·M O Hengartner
Nov 7, 2000·Current Opinion in Cell Biology·S V Ekholm, S I Reed
Mar 23, 2002·Science·Thijn R BrummelkampReuven Agami
Jul 19, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Weimin Zhao, Rui Wang
Nov 1, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Rui Wang
Mar 21, 2003·Biochemical and Biophysical Research Communications·Zhang ChunyuTang Chaoshu
May 10, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Bart Deplancke, H Rex Gaskins
Sep 19, 2003·Antioxidants & Redox Signaling·Rui Wang
Nov 14, 2003·Canadian Journal of Physiology and Pharmacology·Weimin ZhaoRui Wang
Dec 12, 2003·Biochemical Pharmacology·Xianfeng SunRui Wang
Dec 16, 2003·Biochemical and Biophysical Research Communications·Hui YanChaoshu Tang
May 18, 2004·Biochemical and Biophysical Research Communications·Bin GengChaoshu Tang
Sep 15, 2004·European Journal of Pharmacology·Yong-Zhong HouYing-Jin Yuan
Sep 17, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Guangdong YangRui Wang

❮ Previous
Next ❯

Citations

Dec 4, 2009·Proceedings of the National Academy of Sciences of the United States of America·Andreas PapapetropoulosCsaba Szabó
Sep 4, 2010·Antioxidants & Redox Signaling·Stefano M Marino, Vadim N Gladyshev
Oct 9, 2012·Molecular Human Reproduction·Kewen ZhouXiaodong Fu
Aug 30, 2012·Clinical and Experimental Pharmacology & Physiology·Guo-Feng LiJian-Xin Zhang
Mar 10, 2012·Journal of Applied Physiology·Asaf SteinShannon M Bailey
Feb 8, 2011·American Journal of Respiratory Cell and Molecular Biology·Mark M PerryKian Fan Chung
Oct 31, 2008·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Xia ZhaoJun-Bao DU
Jul 25, 2009·Journal of the American Society of Nephrology : JASN·Eelke M BosHarry van Goor
Jan 15, 2014·Biochimica Et Biophysica Acta·Omer KabilRuma Banerjee
Apr 22, 2014·Chemico-biological Interactions·Beata Olas
Sep 20, 2007·Proceedings of the National Academy of Sciences of the United States of America·John W ElrodDavid J Lefer
Oct 25, 2011·Journal of Inherited Metabolic Disease·Carla GiordanoMassimo Zeviani
Jun 15, 2014·Nitric Oxide : Biology and Chemistry·Maria Letizia Lo FaroMatthew Whiteman
Mar 19, 2014·Cellular and Molecular Life Sciences : CMLS·Ping Song, Ming-Hui Zou
Jan 7, 2010·Cardiovascular Research·Guangdong YangRui Wang
Jun 6, 2015·International Journal of Molecular Sciences·Hui GongYunzeng Zou
Dec 3, 2014·Clinica Chimica Acta; International Journal of Clinical Chemistry·Beata Olas
Dec 27, 2015·Pharmacology & Therapeutics·William R DunnRichard E Roberts
Mar 29, 2011·Pharmacological Reports : PR·Bogdan WilińskiMarta Góralska
Jul 31, 2012·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Stefania MerighiPier Andrea Borea
Jan 3, 2012·Biochemical Pharmacology·Mitali ChattopadhyayKhosrow Kashfi
Apr 17, 2010·Biochemical and Biophysical Research Communications·Wang QiaoDu Junbao
Aug 26, 2009·Neurochemistry International·Boon Hian TanJin-Song Bian
Sep 2, 2015·Nitric Oxide : Biology and Chemistry·Serap GurWayne J G Hellstrom
Jun 3, 2009·Journal of Anatomy·Stefanie KrückMartin Scaal
Apr 21, 2009·Journal of Cellular and Molecular Medicine·Matthew Whiteman, Philip K Moore
Nov 8, 2012·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Yang LiuGang Cao
Jan 28, 2010·Cell Biochemistry and Function·David J ElseyGary F Baxter
Jul 16, 2009·Journal of Periodontal Research·J-H ZhangL Chu
Jun 27, 2008·Annales de cardiologie et d'angéiologie·L Rochette, C Vergely

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis