Pro-apoptotic effects of tectorigenin on human hepatocellular carcinoma HepG2 cells.

World Journal of Gastroenterology : WJG
Chun-Ping JiangJun-Hua Wu

Abstract

To investigate the effects of tectorigenin on human hepatocellular carcinoma (HCC) HepG2 cells. Tectorigenin, one of the main components of rhizome of Iris tectorum, was prepared by simple methods, such as extraction, filtration, concentration, precipitation and recrystallization. HepG2 cells were incubated with tectorigenin at different concentrations, and their viability was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Apoptosis was detected by morphological observation of nuclear change, agarose gel electrophoresis of DNA ladder, and flow cytometry with Hoechst 33342, Annexin V-EGFP and propidium iodide staining. Generation of reactive oxygen species was quantified using DCFH-DA. Intracellular Ca(2+) was monitored by Fura 2-AM. Mitochondrial membrane potential was monitored using Rhodamine 123. Release of cytochrome c from mitochondria to cytosol was detected by Western blotting. Activities of caspase-3, -8 and -9 were investigated by Caspase Activity Assay Kit. The viability of HepG2 cells treated by tectorigenin decreased in a concentration- and time-dependent manner. The concentration that reduced the number of viable HepG2 cells by 50% (IC(50)) after 12, 24 and 48 h of incubation was 35...Continue Reading

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Citations

Apr 2, 2014·Journal of Clinical Biochemistry and Nutrition·Tetsuo AdachiHirokazu Hara
Jul 28, 2015·Experimental Eye Research·Keiichi ShibagakiMasatsugu Nakamura
Jun 7, 2013·Immunopharmacology and Immunotoxicology·Shailendra Kapoor
Feb 25, 2018·Cytotechnology·Filiz ÖzdemirZerrin İncesu
Jan 12, 2019·Natural Product Reports·Nawaf Al-Maharik
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Apr 2, 2016·International Journal of Oncology·Yaxiong GuoChengshi Quan
Dec 17, 2020·Medicina·Liang-Tsai YehChih-Jung Chen

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