Pro-inflammatory activation of primary microglia and macrophages increases 18 kDa translocator protein expression in rodents but not humans

Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism
David R OwenCraig S Moore

Abstract

The 18kDa Translocator Protein (TSPO) is the most commonly used tissue-specific marker of inflammation in positron emission tomography (PET) studies. It is expressed in myeloid cells such as microglia and macrophages, and in rodent myeloid cells expression increases with cellular activation. We assessed the effect of myeloid cell activation on TSPO gene expression in both primary human and rodent microglia and macrophages in vitro, and also measured TSPO radioligand binding with 3H-PBR28 in primary human macrophages. As observed previously, we found that TSPO expression increases (∼9-fold) in rodent-derived macrophages and microglia upon pro-inflammatory stimulation. However, TSPO expression does not increase with classical pro-inflammatory activation in primary human microglia (fold change 0.85 [95% CI 0.58-1.12], p = 0.47). In contrast, pro-inflammatory activation of human monocyte-derived macrophages is associated with a reduction of both TSPO gene expression (fold change 0.60 [95% CI 0.45-0.74], p = 0.02) and TSPO binding site abundance (fold change 0.61 [95% CI 0.49-0.73], p < 0.0001). These findings have important implications for understanding the biology of TSPO in activated macrophages and microglia in humans. They are...Continue Reading

References

Sep 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·N S ChandelP T Schumacker
Oct 3, 2001·Journal of Neurovirology·J R LokensgardP K Peterson
Nov 7, 2003·Glia·Josep SauraJoan Serratosa
Mar 17, 2004·Molecular and Cellular Neurosciences·Susanne A KuhnHelmut Kettenmann
Sep 27, 2005·Neurobiology of Disease·Alexander GerhardDavid J Brooks
Mar 21, 2006·Journal of Neuroscience Research·Seok H HongJames G McLarnon
Aug 4, 2007·Glia·Sergio VeigaLuis Miguel Garcia-Segura
Mar 11, 2008·The Journal of Steroid Biochemistry and Molecular Biology·Andres Gottfried-BlackmoreKaren Bulloch
Dec 4, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Caroline LambertJack P Antel
Mar 24, 2009·Annual Review of Immunology·Richard M Ransohoff, V Hugh Perry
Mar 9, 2010·Brain, Behavior, and Immunity·Caroline LambertJack P Antel
Apr 29, 2010·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·David R OwenChristine A Parker
Oct 20, 2011·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·David R OwenJustin P Rubio
Sep 8, 2012·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Erin HarbertsSteven Jacobson
Nov 22, 2012·Best Practice & Research. Clinical Endocrinology & Metabolism·Vassilios Papadopoulos, Walter L Miller
May 17, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Anna M BarronChristian J Pike
May 18, 2013·EMBO Molecular Medicine·Daniel J DaughertyWenbin Deng
Jul 3, 2013·Annals of Neurology·Craig S MooreJack P Antel
Dec 10, 2013·Nature Neuroscience·Oleg ButovskyHoward L Weiner
Jan 7, 2014·Trends in Neurosciences·Amy M Smith, Mike Dragunow
Mar 7, 2014·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Minhua WangWai T Wong
Apr 2, 2014·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Keun-Ryung BaeSeong-Woon Yu
Jun 7, 2014·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Alessandro ColasantiPaul M Matthews
Jun 26, 2014·Nature Reviews. Immunology·Michael T HenekaEicke Latz
Oct 28, 2014·Brain Pathology·Guo-Jun LiuRichard B Banati
Nov 20, 2014·Nature Communications·Richard B BanatiGuo-Jun Liu
Mar 31, 2015·Neurology. Neuroimmunology and Neuroinflammation·Craig S MooreJack P Antel
Apr 1, 2015·Brain : a Journal of Neurology·Mackenzie A Michell-RobinsonCraig S Moore
Sep 20, 2015·Proceedings of the National Academy of Sciences of the United States of America·Christine M SandiegoKelly P Cosgrove

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Citations

Dec 9, 2017·International Review of Psychiatry·Christin SchifaniRomina Mizrahi
Mar 9, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Bieneke JanssenRobert H Mach
Jun 8, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nicolas VignalBenoît Hosten
Dec 20, 2017·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Paul CummingRichard Banati
Oct 20, 2018·Journal of Neurochemistry·Aisling ChaneyHerve Boutin
Jan 10, 2019·International Journal of Molecular Sciences·Stefanie ScheuJudith Alferink
Feb 12, 2019·Psychological Medicine·P Plavén-Sigray, S Cervenka
Jun 24, 2020·Journal of Leukocyte Biology·Idy H T HoXiaodong Liu
Aug 20, 2019·Nature Reviews. Neurology·Paul M Matthews
Aug 18, 2020·Journal of Alzheimer's Disease : JAD·Benjamin B TournierPhilippe Millet
Dec 6, 2018·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Anna Boerwinkle, Beau M Ances
Mar 4, 2020·Neurology. Neuroimmunology and Neuroinflammation·Svetlana BezukladovaLaura Airas
Jul 14, 2020·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Anna H BoerwinkleBeau M Ances
May 8, 2018·European Journal of Nuclear Medicine and Molecular Imaging·Simone L VerweijErik S G Stroes
Apr 19, 2018·Cerebral Cortex·Allyson P MallyaAriel Y Deutch
Jul 28, 2018·Brain : a Journal of Neurology·Melanie DaniPaul Edison
Aug 30, 2018·Cellular and Molecular Life Sciences : CMLS·Niina VuokilaNoora Puhakka
Sep 13, 2019·Current Pharmaceutical Design·Dusica Maysinger, Jeff Ji
Mar 14, 2020·EJNMMI Research·Eric A WoodcockAnsel T Hillmer
Jul 3, 2019·International Journal of Molecular Sciences·Eryn L WerryMichael Kassiou
Aug 28, 2020·Frontiers in Psychology·Ana Carolina Pinheiro CamposRaquel Chacon Ruiz Martinez
Aug 26, 2020·Cells·Benjamin B TournierPhilippe Millet
Aug 4, 2018·Frontiers in Aging Neuroscience·Carmen Hierro-BujalanceMonica Garcia-Alloza
Oct 30, 2019·Journal of Nuclear Cardiology : Official Publication of the American Society of Nuclear Cardiology·Tobias BorchertJames T Thackeray
May 14, 2020·Molecular Psychiatry·Tina NotterJeremy Hall
Jul 22, 2019·International Journal of Molecular Sciences·Vladimir M MilenkovicChristian H Wetzel
Jul 22, 2019·Nature Reviews. Neurology·Fiona BrightJillian J Kril
Jun 26, 2020·Frontiers in Aging Neuroscience·Sara BelloliMaria Carla Gilardi
Aug 21, 2018·Schizophrenia Bulletin·Allyson P Mallya, Ariel Y Deutch
Oct 18, 2020·Cancers·Laura-Marie AmmerPeter Hau
Jan 13, 2021·European Journal of Nuclear Medicine and Molecular Imaging·Erik NutmaBenjamin B Tournier

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Datasets Mentioned

BETA
GM-CSF

Methods Mentioned

BETA
biopsies
ELISA
flow
PCR
Genotyping
Assay
electrophoresis
MDM
lavage

Software Mentioned

GraphPad
Prism
ARRIVE
GraphPad Prism

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