Pro-inflammatory cytokine and high doses of ionizing radiation have similar effects on the expression of NF-kappaB-dependent genes

Cellular Signalling
Patryk JanusPiotr Widlak

Abstract

The NF-κB transcription factors are activated via diverse molecular mechanisms in response to various types of stimuli. A plethora of functions associated with specific sets of target genes could be regulated differentially by this factor, affecting cellular response to stress including an anticancer treatment. Here we aimed to compare subsets of NF-κB-dependent genes induced in cells stimulated with a pro-inflammatory cytokine and in cells damaged by a high dose of ionizing radiation (4 and 10 Gy). The RelA-containing NF-κB species were activated by the canonical TNFα-induced and the atypical radiation-induced pathways in human osteosarcoma cells. NF-κB-dependent genes were identified using the gene expression profiling (by RNA-Seq) in cells with downregulated RELA combined with the global profiling of RelA binding sites (by ChIP-Seq), with subsequent validation of selected candidates by quantitative PCR. There were 37 NF-κB-dependent protein-coding genes identified: in all cases RelA bound in their regulatory regions upon activation while downregulation of RELA suppressed their stimulus-induced upregulation, which apparently indicated the positive regulation mode. This set of genes included a few "novel" NF-κB-dependent speci...Continue Reading

Citations

Aug 1, 2018·International Journal of Molecular Sciences·Christine E HellwegChrista Baumstark-Khan
Mar 21, 2020·Cancers·Elise AasebøMaria Hernandez-Valladares
Apr 24, 2018·Nucleic Acids Research·Hua-Sheng ChiuAndrea Califano
Jan 14, 2020·Pakistan Journal of Biological Sciences : PJBS·Asmaa Magdy ZaazaaNadia Noble-Daoud Aniss
Dec 31, 2019·Oxidative Medicine and Cellular Longevity·Kengo WagaIkuo Kashiwakura
Dec 25, 2019·Nature Immunology·Maria Esperanza Rodriguez-RuizLorenzo Galluzzi
May 1, 2021·Life·Jayan D M Senevirathna, Shuichi Asakawa
Sep 30, 2021·International Journal of Radiation Biology·Sally A Amundson

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