Pro-inflammatory cytokines and gelatinases in climatic droplet keratopathy

Investigative Ophthalmology & Visual Science
Juha M HolopainenHoracio M Serra

Abstract

Climatic droplet keratopathy (CDK) is a degenerative disease of the cornea with possible involvement from matrix metalloproteinases (MMPs). Therefore, the authors investigated histologic distribution, levels, and molecular forms of MMP-2 and MMP-9, as well as tear fluid levels of MMPs and cytokines in CDK patients. They additionally examined UV-B-irradiation effect on production of gelatinases and cytokines by human corneal epithelial (HCE) cell culture model. Tears were collected from 20 unrelated individuals (10 with CDK and 10 controls). CDK affected corneas were haematoxylin-eosin stained and the presence and distribution of MMP-2 and MMP-9 was examined using immunohistochemistry. Gelatinases and cytokine secretion was measured in tears and supernatants from UV-B-exposed HCEs by immunoblotting, gelatin zymography, and protein array, respectively. MMP-2 and MMP-9 values were significantly higher in tears collected from CDK patients than healthy controls and were accompanied by pro-inflammatory cytokine secretion. Immunohistochemistry showed that MMP-2 was expressed at the basement membrane zone in both control and affected corneas, but also marked the edges of the granular CDK deposits; MMP-9 expression was restrained to bas...Continue Reading

Citations

Jun 20, 2013·Colloids and Surfaces. B, Biointerfaces·Kofi Asare-AddoHassan Larhrib
Jan 30, 2015·Acta Ophthalmologica·Horacio M SerraJulio A Urrets-Zavalía
Dec 10, 2016·Photochemistry and Photobiology·Naomi C DelicGary M Halliday
Apr 13, 2017·Journal of Cellular Biochemistry·Maria Fernanda SuarezHoracio M Serra
Feb 28, 2020·Investigative Ophthalmology & Visual Science·P Ewen King-SmithPatrice Tankam
Feb 19, 2020·Eye & Contact Lens·Emery C JamersonYasmine M El Sayed

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