Pro-inflammatory properties of H-ferritin on human macrophages, ex vivo and in vitro observations.

Scientific Reports
Piero RuscittiRoberto Giacomelli

Abstract

Ferritin is an iron-binding molecule, which comprises 24 subunits, heavy (FeH) and light (FeL) subunits, suggested to have a pathogenic role by the 'hyperferritinemic syndrome'. In this work, we tested (1) FeH and FeL in bone marrow (BM) and sera in patients with macrophage activation syndrome (MAS); (2) pro-inflammatory effects of ferritin, FeL, and FeH on macrophages; (3) ability of FeH-stimulated macrophages to stimulate the proliferation of peripheral blood mononuclear cells (PBMCs); (4) production of mature IL-1β and IL-12p70 in extracellular compartments of FeH-stimulated macrophages. Immunofluorescence analysis and liquid chromatography mass spectrometry (LC-MS/MS) based proteomics were performed to identify FeL and FeH in BM and sera, respectively, in the same patients. Macrophages were stimulated with ferritin, FeH, and FeL to assess pro-inflammatory effects by RT-PCR and western blot. The proliferation of co-cultured PBMCs with FeH-stimulated macrophages was tested. Immunofluorescence showed an increased FeH expression in BMs, whereas LC-MS/MS identified that FeL was mainly represented in sera. FeH induced a significant increase of gene expressions of IL-1β, IL-6, IL-12, and TNF-α, more marked with FeH, which also sti...Continue Reading

References

May 31, 1990·Biochemical and Biophysical Research Communications·Y WeiF M Torti
Oct 6, 2005·The Journal of Experimental Medicine·Thomas T ChenMichael R Daws
Jan 15, 2008·Journal of Autoimmunity·Stefania RecalcatiGaetano Cairo
Feb 26, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Richard G RuddellGrant A Ramm
May 12, 2009·Immunological Reviews·Roselynn Rodriguez-ManzanetDale T Umetsu
Mar 17, 2010·The Journal of Rheumatology·Junko Maruyama, Shigeko Inokuma
Mar 23, 2010·Biochimica Et Biophysica Acta·Wei WangSuzy V Torti
Jul 21, 2011·Nature Immunology·Yinon Ben-Neriah, Michael Karin
Apr 27, 2012·Genetics and Molecular Research : GMR·S ChuchawankulY Poovorawan
Jul 21, 2012·Nature Immunology·Dario A A Vignali, Vijay K Kuchroo
Apr 17, 2013·The Journal of Rheumatology·Raffaele StrippoliFabrizio De Benedetti
Dec 3, 2013·Lancet·Manuel Ramos-CasalsXavier Bosch
Jan 18, 2014·Clinical Rheumatology·Petros EfthimiouBella Mehta
Mar 25, 2014·Autoimmunity Reviews·Mathieu Gerfaud-ValentinPascal Sève
Feb 3, 2015·Trends in Cell Biology·Sivapriya K VanajaKatherine A Fitzgerald
Apr 12, 2015·Annual Review of Immunology·Chen VarolSteffen Jung
Mar 25, 2016·Nature Reviews. Rheumatology·Alexei A GromFabrizio De Benedetti
May 8, 2016·Cell·Vijay A K Rathinam, Katherine A Fitzgerald
Jun 14, 2016·Nature Reviews. Immunology·Petr Broz, Vishva M Dixit
Jul 1, 2016·Scandinavian Journal of Rheumatology·E D BatuU Kalyoncu
Jan 18, 2017·Pediatric Rheumatology Online Journal·Claudia BracagliaFabrizio De Benedetti
Sep 6, 2017·Expert Review of Clinical Immunology·Piero RuscittiRoberto Giacomelli
Oct 13, 2017·Clinical and Experimental Immunology·K SharifY Shoenfeld
Feb 20, 2018·Immunology·Samanta C FunesAlexis M Kalergis
Aug 6, 2018·Journal of Autoimmunity·Roberto GiacomelliYehuda Shoenfeld
Oct 9, 2018·Expert Review of Clinical Immunology·Piero Ruscitti, Roberto Giacomelli
Feb 26, 2019·Frontiers in Immunology·Abolfazl ZarjouSubhashini Bolisetty

❮ Previous
Next ❯

Citations

Jan 26, 2021·Drug Discovery Today·Prakash VasanthiDharmalingamRajarajan A Thandavarayan
Apr 22, 2021·Metallomics : Integrated Biometal Science·Marina PlaysRaphaël Rodriguez
Aug 15, 2021·Seminars in Hematology·Domenico GirelliAlice Vianello

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsies
ELISA
reversed phase chromatography
PCR
Protein Assay

Software Mentioned

GraphPad Prism
NIHimageJ
Proteome Discoverer
Mascot
Xcalibur

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
Ahmed K SaidStephen R Porter
Annals of Agricultural and Environmental Medicine : AAEM
Aleksandra KasperczykSławomir Kasperczyk
Dermatology : International Journal for Clinical and Investigative Dermatology
M MoermanH Vermeersch
The New Phytologist
Richard J Simpson, Graham D Bonnett
© 2021 Meta ULC. All rights reserved