Pro-oxidant and cytotoxic activities of atractylenolide I in human promyeloleukemic HL-60 cells

Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
Ching-Chiung WangWen-Chi Hou

Abstract

The dried rhizome of Bai Zhu (Atractylodes ovata) is widely used as a Chinese herbal medicine. Two sesquiterpenolides of similar structures (atractylenolide I, AT-I; atractylenolide III, AT-III) were isolated from dried rhizome of Atractylodes ovata. Incubation of AT-I with recombinant human Cu,Zn-superoxide dismutase (rhCu,Zn-SOD) resulted in rhCu,Zn-SOD fragmentations and Zn releases. However, these were not observed in the AT-III reaction. The AT-1 showed dose-dependent cytotoxic activities (7.5, 15, and 30 microg/ml) on the human promyeloleukemic HL-60 cells while AT-III did not, and the IC50 of the former being 10.6 microg/ml (corresponding to 46 microM) on 12 h-treated cells. The results of DNA ladder and DNA contents in sub-G1 type revealed that AT-I induced apoptosis in human promyeloleukemic HL-60 cells. The cytotoxic and pharmacological mechanisms of AT-I against human promyeloleukemic HL-60 cells was investigated. The AT-I appeared to exhibit both pro-oxidant and antioxidant properties after an ESR spectrometer was used to detect hydroxyl radical productions in vitro and flow cytometry to detect intracellular ROS productions in AT-I treated cells. The AT-1 also showed dose-dependent Cu,Zn-SOD inhibitory activity in H...Continue Reading

References

Dec 1, 1979·Chemical & Pharmaceutical Bulletin·K EndoH Fujimura
Jan 1, 1991·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·H MatsudaY Tamai
Nov 1, 1971·Analytical Biochemistry·W WoodburyM A Stahman
Nov 1, 1971·Analytical Biochemistry·C Beauchamp, I Fridovich
Sep 1, 1983·Journal of Natural Products·Y KisoH Hikino
Feb 1, 1993·Biological & Pharmaceutical Bulletin·T SakuraiY Kano
Mar 3, 1997·FEBS Letters·A GormanT G Cotter
Jun 1, 1997·Journal of Pharmacological and Toxicological Methods·R T AllenD K Agrawal
Feb 14, 2002·Molecular Immunology·Shugo UedaJunji Yodoi
Mar 27, 2002·Planta medica·Ching-Chiung WangLing-Ling Yang
Nov 9, 2002·European Journal of Biochemistry·Paulo FilipePatrice Morlière
Aug 12, 2004·Free Radical Biology & Medicine·Beatriz AlvarezRafael Radi
Apr 23, 2005·Journal of Natural Products·Jorge TrianaJaime Bermejo
Jun 15, 2005·Annual Review of Biochemistry·Joan Selverstone ValentineSoshanna Zittin Potter
Sep 27, 2005·Toxicology Letters·Feng-Yih YuBiing-Hui Liu
Oct 4, 2005·Free Radical Biology & Medicine·Katsuhisa SakanoShosuke Kawanishi

❮ Previous
Next ❯

Citations

Oct 3, 2008·Evidence-based Complementary and Alternative Medicine : ECAM·Yi LiuGenquan Qiu
Jul 30, 2011·Evidence-based Complementary and Alternative Medicine : ECAM·Kun-Teng WangChing-Chiung Wang
Oct 22, 2011·Journal of Pharmaceutical and Biomedical Analysis·Yujuan LiBo Chen
Feb 9, 2010·Journal of Ethnopharmacology·Ho Gyoung KimTae Woong Kim
Apr 10, 2009·European Journal of Pharmacology·Changhe WangLangchong He
May 22, 2010·The Journal of Pharmacy and Pharmacology·Kun-Teng WangChing-Chiung Wang
Nov 29, 2011·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Judith SinghuberSteffen Hering
Jul 30, 2014·Asian Pacific Journal of Tropical Medicine·Nut KoonrungsesomboonJuntra Karbwang
Jun 20, 2014·Biochemical and Biophysical Research Communications·Li MaLei Ni
Apr 21, 2016·Journal of Agricultural and Food Chemistry·Yuh-Hwa LiuWen-Chi Hou
Feb 9, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Changhe WangLangchong He
Jun 20, 2018·BMC Pharmacology & Toxicology·Pimradasiri SrijiwangsaKesara Na-Bangchang
Jul 2, 2021·Journal of Traditional and Complementary Medicine·Kesara Na-BangchangJuntra Karbwang
Jul 17, 2021·Archives of Pharmacal Research·Mao DengXiaofang Li

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Ataxia telangiectasia (MDS)

Ataxia telangiectasia is a rare neurodegenerative diseases caused by defects in the ATM gene, which is involved in DNA damage recognition and repair pathways. Here is the latest research on this autosomal recessive disease.