Probable impact of age and hypoxia on proliferation and microRNA expression profile of bone marrow-derived human mesenchymal stem cells

PeerJ
Norlaily Mohd AliTunku Kamarul

Abstract

Decline in the therapeutic potential of bone marrow-derived mesenchymal stem cells (MSC) is often seen with older donors as compared to young. Although hypoxia is known as an approach to improve the therapeutic potential of MSC in term of cell proliferation and differentiation capacity, its effects on MSC from aged donors have not been well studied. To evaluate the influence of hypoxia on different age groups, MSC from young (<30 years) and aged (>60 years) donors were expanded under hypoxic (5% O2) and normal (20% O2) culture conditions. MSC from old donors exhibited a reduction in proliferation rate and differentiation potential together with the accumulation of senescence features compared to that of young donors. However, MSC cultured under hypoxic condition showed enhanced self-renewing and proliferation capacity in both age groups as compared to normal condition. Bioinformatic analysis of the gene ontology (GO) and KEGG pathway under hypoxic culture condition identified hypoxia-inducible miRNAs that were found to target transcriptional activity leading to enhanced cell proliferation, migration as well as decrease in growth arrest and apoptosis through the activation of multiple signaling pathways. Overall, differentially ...Continue Reading

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Citations

Dec 19, 2016·European Journal of Cell Biology·Sajjad SisakhtnezhadHassan Akrami
Nov 9, 2016·World Journal of Stem Cells·Miruna Mihaela MicheuOana-Claudia Deleanu
Mar 13, 2019·International Journal of Molecular Sciences·Cristina Mas-BarguesConsuelo Borrás
Jan 30, 2019·Cell Death & Disease·Ejlal Abu-El-RubSanjiv Dhingra
Dec 2, 2017·The International Journal of Artificial Organs·Sophie von NathusiusNikolaus Thierfelder
Jul 2, 2019·High Altitude Medicine & Biology·Natasja Leth BergholtCasper Bindzus Foldager
Oct 10, 2020·World Journal of Stem Cells·Qing-Shu MengXiao-Ting Liang
Apr 6, 2021·Frontiers in Cell and Developmental Biology·Jingyi CaiZhihe Zhao

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Datasets Mentioned

BETA
GSE67630

Methods Mentioned

BETA
PCR
chip
PCA

Software Mentioned

miRDB
DAVID
CLC Genomic Workbench
geNorm
ImageJ

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