Probes for biomolecules detection based on RET-enhanced fluorescence polarization

Biosensors & Bioelectronics
Dahai RenZheng You

Abstract

Fluorescent probes based on the principle of resonance energy transfer (RET) or the principle of fluorescence polarization (FP) are already used to detect biomolecules independently. However, there were no in-depth studies about the impact of RET on FP. Also, very few studies gave a comprehensive analysis on how to effectively design such a fluorescent probe. Based on the principle of resonance energy transfer (RET), we constructed fluorescent probes (SA-488-sub-nanogold) using streptavidin labeled Alexa488 (SA-488), nanogold and biotinylated substrate peptide (biotin-subpeptide). The influence of the structure and the ingredients of the substrate peptide were discussed. After SA-488 was combined with the biotin-subpeptide and the nanogold, its fluorescence intensity (FI) would be suppressed due to the energy transfer, leading to an increase in its volume and mass. The suppression of the FI led to a decrease in SA-488's effective concentration, and the increase in the volume or mass prolonged the SA-488's rotational relaxation time. Both changes increased SA-488's polarization in the solution. Therefore, the FP performance of the probe is enhanced by the RET. Using the probe, trypsin and biotin were detected by the change in bo...Continue Reading

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Citations

May 3, 2016·The Journal of Physical Chemistry. B·Yue HuangFiorenzo Vetrone
Mar 10, 2017·Drug Development and Industrial Pharmacy·Jéssica Bassi da SilvaMarcos Luciano Bruschi
Jan 29, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Nobutoshi OtaYo Tanaka
Mar 21, 2019·Royal Society Open Science·Nobutoshi OtaYo Tanaka
Apr 26, 2017·Clinical Epigenetics·Sheraz Gul
Jun 3, 2018·Sensors·Yixing GouChangku Sun

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