Probes for hidden hyperdiploidy in acute lymphoblastic leukaemia

Genes, Chromosomes & Cancer
Anthony V MoormanL M Secker-Walker

Abstract

The detection of hyperdiploidy (clones with >46 chromosomes) in the bone marrow of patients with acute lymphoblastic leukaemia (ALL) is important because of the prognostic impact of this finding. The high hyperdiploid (HeH) subgroup with 51-68 chromosomes is associated with the best outcome, followed by the low hyperdiploid (HeL) subgroup with 47-50 chromosomes and the triploid/tetraploid (TT) subgroup with >68 chromosomes, which do less well. We present a strategy for the use of fluorescence in situ hybridization (FISH) with chromosome-specific probes to detect hyperdiploidy in interphase cells and to assign cases to a ploidy subgroup. By using a model population of 252 cases, it was seen that ten chromosomes (X, 4, 6, 8, 10, 14, 16, 18, 20, and 21) used in particular combinations and applied in a step-wise manner enabled the detection of 94% of hyperdiploid cases and gave an accurate prediction of ploidy subgroup in 96% of these cases. The detection and classification of each case required the use of four to six probes over two or three steps. Confirmation that this strategy will achieve this level of detection in other hyperdiploid populations was demonstrated by using 250 published karyotypes. This strategy has an applicati...Continue Reading

References

Oct 1, 1990·Cancer Genetics and Cytogenetics·L M Secker-Walker
Nov 1, 1990·The American Journal of Medicine·R WaltersE J Freireich
Jul 15, 1989·Cancer Genetics and Cytogenetics·C D BloomfieldJ D Rowley
Oct 1, 1993·Genes, Chromosomes & Cancer·S P RomanaR Berger
Oct 1, 1996·Leukemia & Lymphoma·C A SmithC M Rooney

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Citations

May 30, 2001·Cancer Genetics and Cytogenetics·A V MoormanG J Morgan
Mar 1, 1997·Cancer Genetics and Cytogenetics·R Berger
Jun 8, 2001·Archives of Medical Research·P Pérez-VeraS Frías
Aug 28, 2002·Reviews in Clinical and Experimental Hematology·Christine J Harrison, Letizia Foroni
Apr 28, 2004·Journal of Pediatric Hematology/oncology·Patricia Pérez-VeraRocío Ortíz
Dec 1, 2001·Journal of Clinical Pathology·N Bown
Aug 26, 1998·Molecular Pathology : MP·J J WatersC P Gould
May 26, 1999·Leukemia & Lymphoma·H Avet-Loiseau
Mar 14, 2007·Archives of Medical Research·Achandira Muthappa UdayakumarJohn Alexander Raeburn
Apr 23, 2004·Leukemia & Lymphoma·Nathalie Douet-GuilbertMarc De Braekeleer
May 6, 2009·Genes, Chromosomes & Cancer·Kajsa Paulsson, Bertil Johansson
Apr 29, 1999·Medical and Pediatric Oncology·K HeinonenM Perkkiö
Apr 1, 1997·The Journal of Pathology·K Q Wolfe, C S Herrington
Dec 29, 2017·Cancers·Natalia Vargas-RondónMilena Rondón-Lagos
Apr 18, 2002·Leukemia·M S Jabber Al-ObaidiUNKNOWN Medical Research Council Adult Leukaemia Working Party
Jul 20, 2006·Pediatric Hematology and Oncology·Hsiu-Hao ChangDong-Tsamn Lin
Nov 30, 2012·International Journal of Laboratory Hematology·S HutspardolS Hongeng
Mar 16, 2006·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Melinda C AldrichPatricia A Buffler

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