Probing ADP Induced Aggregation Kinetics During Platelet-Nanoparticle Interactions: Functional Dynamics Analysis to Rationalize Safety and Benefits

Frontiers in Bioengineering and Biotechnology
Souvik K BandyopadhyaySuryyani Deb

Abstract

Platelets, one of the most sensitive blood cells, can be activated by a range of external and internal stimuli including physical, chemical, physiological, and/or non-physiological agents. Platelets need to respond promptly during injury to maintain blood hemostasis. The time profile of platelet aggregation is very complex, especially in the presence of the agonist adenosine 5'-diphosphate (ADP), and it is difficult to probe such complexity using traditional linear dose response models. In the present study, we explored functional analysis techniques to characterize the pattern of platelet aggregation over time in response to nanoparticle induced perturbations. This has obviated the need to represent the pattern of aggregation by a single summary measure and allowed us to treat the entire aggregation profile over time, as the response. The modeling was performed in a flexible manner, without any imposition of shape restrictions on the curve, allowing smooth platelet aggregation over time. The use of a probabilistic framework not only allowed statistical prediction and inference of the aggregation signatures, but also provided a novel method for the estimation of higher order derivatives of the curve, thereby allowing plausible ...Continue Reading

References

Mar 1, 1994·Canadian Journal of Physiology and Pharmacology·M A Packham
Feb 5, 2002·Archives of Pathology & Laboratory Medicine·Kandice Kottke-Marchant, George Corcoran
May 10, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Robert Flaumenhaft
Feb 3, 2004·The Journal of Clinical Investigation·Robert T Dorsam, Satya P Kunapuli
Sep 15, 2005·British Journal of Pharmacology·Anna RadomskiMarek W Radomski
Dec 22, 2005·Frontiers in Bioscience : a Journal and Virtual Library·Swaminathan Murugappa, Satya P Kunapuli
Feb 23, 2008·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Catherine P M Hayward
Dec 17, 2008·Environmental Health Perspectives·Jorina GeysPeter H M Hoet
Jun 24, 2009·ACS Nano·Siddhartha ShrivastavaDebabrata Dash
Jan 1, 2009·Electronic Journal of Statistics·David RuppertRaymond J Carroll
Feb 12, 2011·Nanomedicine : Nanotechnology, Biology, and Medicine·Suryyani DebUtpal Chaudhuri
Oct 29, 2011·Blood Cells, Molecules & Diseases·Suryyani DebPartha Sardar
Jan 11, 2013·Blood·Iris M De CuyperLaura Gutiérrez
Mar 4, 2015·Vascular Health and Risk Management·Rita PanicciaRosanna Abbate

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