Probing conformations of the beta subunit of F0F1-ATP synthase in catalysis

Biochemical and Biophysical Research Communications
Tomoko MasaikeMasasuke Yoshida

Abstract

A subcomplex of F0F1-ATP synthase (F0F1), alpha3beta3gamma, was shown to undergo the conformation(s) during ATP hydrolysis in which two of the three beta subunits have the "Closed" conformation simultaneously (CC conformation) [S.P. Tsunoda, E. Muneyuki, T. Amano, M. Yoshida, H. Noji, Cross-linking of two beta subunits in the closed conformation in F1-ATPase, J. Biol. Chem. 274 (1999) 5701-5706]. This was examined by the inter-subunit disulfide cross-linking between two mutant beta(I386C)s that was formed readily only when the enzyme was in the CC conformation. Here, we adopted the same method for the holoenzyme F0F1 from Bacillus PS3 and found that the CC conformation was generated during ATP hydrolysis but barely during ATP synthesis. The experiments using F0F1 with the epsilon subunit lacking C-terminal helices further suggest that this difference is related to dynamic nature of the epsilon subunit and that ATP synthesis is accelerated when it takes the pathway involving the CC conformation.

References

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Citations

Feb 3, 2011·Biotechnology Letters·Kiyotaka Y HaraKuniki Kino
Nov 18, 2008·Nature Structural & Molecular Biology·Tomoko MasaikeTakayuki Nishizaka
Feb 16, 2010·The Journal of Biological Chemistry·Ei-ichiro SaitaMasasuke Yoshida
Feb 25, 2011·The Journal of Biological Chemistry·Hiroki KonnoToru Hisabori
Jan 19, 2012·The Journal of Biological Chemistry·Naoki SogaToshiharu Suzuki
Aug 19, 2015·Structure·Florian HauerHolger Stark
May 25, 2011·The FEBS Journal·Naoki SogaToshiharu Suzuki
May 10, 2019·The Journal of Biological Chemistry·Kosuke InabeToru Hisabori
Sep 29, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Edgar Morales-RíosJosé J García-Trejo
Feb 14, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Mariel Zarco-ZavalaJosé J García-Trejo
Nov 10, 2006·The Journal of Biological Chemistry·Boris A FenioukMasasuke Yoshida
Apr 2, 2011·The Journal of Biological Chemistry·Toshiharu SuzukiMasasuke Yoshida
Dec 16, 2020·Biochimica Et Biophysica Acta. Bioenergetics·Owen D JarmanJudy Hirst
Apr 24, 2021·Biochemical Society Transactions·Anthony Cheuk, Thomas Meier
May 17, 2006·Biochimica Et Biophysica Acta·Boris A FenioukMasasuke Yoshida

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