PMID: 2105397Jan 1, 1990Paper

Probing DNA structure and function with a multi-wavelength fluorescence confocal laser microscope

Journal of Microscopy
D J Arndt-JovinT M Jovin

Abstract

Three levels of organization in DNA structure in the interphase cell nucleus are assessed by confocal laser scanning microscopy: (i) the conformational state of the double helix; (ii) the distribution of eu- and heterochromatin; and (iii) the localization of replication complexes throughout S phase. Multi-parameter measurements were carried out in each optical section using two laser sources and combined stereoscopic reconstructions were used to assess the co-localization of nuclear components. DNA is highly polymorphic and can adopt a variety of different helical conformations as well as unusual structures (curved, cruciform, multi-stranded). We have assessed by laser scanning microscopy the presence of left-handed Z-DNA in polytene chromosomes of Diptera as well as the spatio-temporal distribution of Z-DNA binding proteins in whole-mount Drosophila embryos and ovaries. We have determined the 3-D distribution of replication sites relative to heterochromatin regions, nucleoli and nuclear membrane by using short pulses of BrdU incorporation in synchronized mouse and human fibroblasts. Replication sites were visualized with a monoclonal anti-BrdU antibody combined with DNA fluorescent staining and antibody labelling of nuclear la...Continue Reading

References

Mar 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A V CarranoM A Van Dilla
Jan 1, 1986·Annals of the New York Academy of Sciences·G J BrakenhoffN Nanninga
Jan 1, 1989·Methods in Cell Biology·D J Arndt-Jovin, T M Jovin
Jan 1, 1989·Methods in Cell Biology·R Y Tsien
Jan 1, 1989·Annual Review of Biophysics and Biophysical Chemistry·T M Jovin, D J Arndt-Jovin
Jan 1, 1986·Annual Review of Biophysics and Biophysical Chemistry·W G NelsonD S Coffey
Jun 2, 1988·Nature·O N VoloshinM D Frank-Kamenetskii
Jul 25, 1988·Nucleic Acids Research·N B Ramsing, T M Jovin
Jul 29, 1988·Science·J H van de SandeT M Jovin
Sep 1, 1987·Journal of Microscopy·H J Gundersen, E B Jensen
Apr 1, 1987·The Journal of Cell Biology·G R BrightD L Taylor
Jan 1, 1985·Cytometry·P N Dean
Oct 18, 1985·Science·D J Arndt-JovinT M Jovin
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·S A Latt
Oct 1, 1983·Journal of Biomolecular Structure & Dynamics·T M JovinF Eckstein
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·D J Arndt-JovinT M Jovin
Jan 11, 1984·Nucleic Acids Research·K WatanabeK Iso
Feb 1, 1984·Journal of Microscopy·I J Cox

❮ Previous
Next ❯

Citations

Jan 1, 1992·Cytometry·C Humbert, Y Usson
Aug 1, 1994·Trends in Pharmacological Sciences·R G King, P M Delaney
Apr 1, 1992·Biophysical Journal·M LudwigR J Hartzman
Dec 22, 1999·Archives of Histology and Cytology·P GobbiG Mazzotti
Jan 1, 1992·Analytical Biochemistry·T Hartmann, A S Verkman
Aug 2, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Luigi F AgnatiDiego Guidolin
Jul 5, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Akira MatsunoR Yoshiyuki Osamura
May 5, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Akira MatsunoR Yoshiyuki Osamura
Mar 1, 1993·Journal of Microscopy·E M M MandersJ A Aten
Feb 1, 1991·Genetic Analysis, Techniques and Applications·P LichterD C Ward

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.