Probing electric fields in proteins in solution by NMR spectroscopy

Proteins
Mathias A S HassJens J Led

Abstract

Electric fields generated in native proteins affect almost every aspect of protein function. We present a method that probes changes in the electric field at specific locations within a protein. The method utilizes the dependence of the amide (1)H and (15)N NMR chemical shifts on electric charges in proteins. Charges were introduced at different positions in the blue copper protein plastocyanin, by protonation of side chains or by substitution of the metal ion. It is found that the associated chemical shift perturbations (CSPs) stem mainly from long-range electric field effects caused by the change in the electric charge. It is demonstrated that the CSPs can be used to estimate the dielectric constant at different locations in the protein, estimate the nuclear shielding polarizability, or position charges in proteins.

References

Nov 20, 1986·Journal of Molecular Biology·J M GussH C Freeman
Nov 1, 1986·Biopolymers·M K Gilson, B H Honig
Jun 19, 1998·Current Opinion in Structural Biology·A Warshel, A Papazyan
Jul 20, 2001·Methods in Enzymology·D S Wishart, D A Case
Feb 13, 2003·Journal of the American Chemical Society·Cyril DominguezAlexandre M J J Bonvin
May 27, 2003·Journal of Biomolecular NMR·Stephen NealDavid S Wishart
Jan 30, 2004·Acta Crystallographica. Section D, Biological Crystallography·Megan MaherAnthony G Wedd
Aug 12, 2004·Chemical Reviews·Arthur G Palmer
Feb 16, 2006·Magnetic Resonance in Chemistry : MRC·Malene Ringkjøbing JensenJens J Led
May 23, 2006·Journal of Inorganic Biochemistry·David M LeMasterGriselda Hernández
Aug 25, 2006·Acta Crystallographica. Section D, Biological Crystallography·Lars SchmidtPernille Harris
Oct 3, 2006·Biophysical Journal·Janet S AndersonGriselda Hernández

❮ Previous
Next ❯

Citations

Jun 18, 2009·Journal of Biomolecular NMR·Mathias A S HassJens J Led
Sep 29, 2011·Journal of Biomolecular NMR·Lawrence P McIntoshJens E Nielsen
Aug 22, 2013·Progress in Nuclear Magnetic Resonance Spectroscopy·Mike P Williamson
Apr 29, 2010·Analytical Chemistry·Vitor H PominJames H Prestegard
Mar 24, 2011·Future Medicinal Chemistry·Predrag Kukić, Jens Erik Nielsen
Apr 25, 2013·Progress in Nuclear Magnetic Resonance Spectroscopy·Quentin Kaas, David J Craik
Sep 6, 2014·Journal of the American Chemical Society·Liaoyuan AnLishan Yao
Jul 12, 2013·Chembiochem : a European Journal of Chemical Biology·Gerd NielsenHarald Schwalbe
Aug 18, 2010·Proteins·Jennifer H TomlinsonMike P Williamson
Feb 7, 2014·Protein Science : a Publication of the Protein Society·Zheng Cao, James U Bowie
Nov 10, 2009·Proteins·Damien FarrellJens Erik Nielsen
Mar 10, 2015·Annual Review of Biophysics·Mathias A S Hass, Frans A A Mulder
Aug 26, 2016·Biophysical Journal·Luciano A AbriataMatteo Dal Peraro
Oct 16, 2013·Journal of the American Chemical Society·Predrag KukicJens Erik Nielsen
Jan 20, 2018·Physical Review. E·Christopher W WahleGeorge M Thurston
Jul 25, 2021·Proceedings of the National Academy of Sciences of the United States of America·Frans A A Mulder
Sep 16, 2010·The Journal of Physical Chemistry. a·Matthew R Tarasek, James G Kempf
Sep 16, 2021·ChemMedChem·Sven BrüschweilerMoriz Mayer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.