PMID: 9189009Jan 1, 1997Paper

Probing for cerebrospinal fluid antibody specificities by a panel of random peptide libraries

Autoimmunity
A DybwadM Sioud

Abstract

In a state of health the central nervous system is nearly devoid of macrophages, T and B cells. However, such cells are required to initiate the inflammation associated with multiple sclerosis (MS). An important issue in understanding the pathogenesis of such conditions is to fully define the T and B cell specificities. Using random peptide libraries, we have analysed the cerebrospinal fluid antibody specificities within one oligoclonal "band" from a patient with MS. The selected peptides from a 6-mer library revealed two peptide motifs S (S/T/Q) (R/S) (N/G) FP and PRn (G/P) FF. Interestingly, the first motif was also selected from a 15-mer library, while the second motif was selected from the 9-mer library. In addition, distinct but structurally related peptides to the mentioned motifs were also selected. Surprisingly, a SwissProt search with these motifs revealed a significant linear homology with collagen proteins, the 68 kDa neurofilament protein, versican and other proteins from viruses such as herpes simplex virus, human cytomegalovirus and human papillomavirus. Thus, the observation that antibodies present within one oligoclonal immunoglobulin band would recognize peptides, some that might be related by only the structur...Continue Reading

References

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Citations

Jul 17, 1998·Journal of Neurology, Neurosurgery, and Psychiatry·K H RandK M Heilman
Apr 28, 2009·Autoimmunity Reviews·Klaartje SomersVeerle Somers
Apr 8, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·A J ReadM W Lightowlers
Mar 7, 2006·Biopolymers·Maria Claudia Alcaro, Anna Maria Papini
Dec 22, 1999·Clinical Immunology : the Official Journal of the Clinical Immunology Society·C Jolivet-ReynaudB Mandrand
Jan 5, 2000·Multiple Sclerosis : Clinical and Laboratory Research·S MatàH Link
Jun 7, 2003·Scandinavian Journal of Immunology·A DybwadM Zouali

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